Molecular characterization of the AtCXE8 gene, which promotes resistance to Botrytis cinerea infection

Sanghyeob Lee, Seongbin Hwang, Yong Weon Seo, Woong Bae Jeon, Boung Jun Oh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The relationships of sterase- and lipase-like proteins with three signaling molecules (salicylic acid, jasmonic acid, and ethylene) expressed during plant-pathogen interactions were studied. We isolated two carboxylesterase (AtCXE) genes, AtCXE8 and AtCXE9, from Arabidopsis thaliana. The AtCXE8 and AtCXE9 proteins possess carboxylesterase motifs (-GXSXG-) and catalytic triads (Ser, Asp, and His). We demonstrated that recombinant AtCXE8 and AtCXE9 proteins have both enzymatic activity and specific activity for p-nitrophenyl butyrate (C4) in vitro. Moreover, the enzymatic activity of recombinant AtCXE8 was twofold higher than that of AtCXE9. To gain a better understanding of the endogenous role of the AtCXE8 gene in Arabidopsis, we identified an enhancer trap T-DNA mutant (AtCXE8_KO) and used it to show that the AtCXE8 gene was induced in response to fungal infection. AtCXE8_KO plants were also more susceptible to infections than wild-type Col-0 plants. Moreover, overexpression of the AtCXE8 gene in transgenic Arabidopsis plants led to enhanced disease resistance against B. cinerea. Taken together, our data indicate that AtCXE8 plays a role in promoting resistance to fungal invasion.

Original languageEnglish
Pages (from-to)109-119
Number of pages11
JournalPlant Biotechnology Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

Fingerprint

Botrytis
Botrytis cinerea
carboxylesterase
Arabidopsis
Carboxylesterase
Infection
infection
Genes
enhancer elements
gene overexpression
genes
proteins
biological resistance
jasmonic acid
butyrates
salicylic acid
plant pathogens
Proteins
Disease Resistance
disease resistance

Keywords

  • Abiotic stress
  • Botrytis cinerea
  • Carboxylesterase
  • Disease resistance

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology

Cite this

Molecular characterization of the AtCXE8 gene, which promotes resistance to Botrytis cinerea infection. / Lee, Sanghyeob; Hwang, Seongbin; Seo, Yong Weon; Jeon, Woong Bae; Oh, Boung Jun.

In: Plant Biotechnology Reports, Vol. 7, No. 1, 01.01.2013, p. 109-119.

Research output: Contribution to journalArticle

Lee, Sanghyeob ; Hwang, Seongbin ; Seo, Yong Weon ; Jeon, Woong Bae ; Oh, Boung Jun. / Molecular characterization of the AtCXE8 gene, which promotes resistance to Botrytis cinerea infection. In: Plant Biotechnology Reports. 2013 ; Vol. 7, No. 1. pp. 109-119.
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