Molecular characterization of the AtCXE8 gene, which promotes resistance to Botrytis cinerea infection

Sanghyeob Lee, Seongbin Hwang, Yong Weon Seo, Woong Bae Jeon, Boung Jun Oh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The relationships of sterase- and lipase-like proteins with three signaling molecules (salicylic acid, jasmonic acid, and ethylene) expressed during plant-pathogen interactions were studied. We isolated two carboxylesterase (AtCXE) genes, AtCXE8 and AtCXE9, from Arabidopsis thaliana. The AtCXE8 and AtCXE9 proteins possess carboxylesterase motifs (-GXSXG-) and catalytic triads (Ser, Asp, and His). We demonstrated that recombinant AtCXE8 and AtCXE9 proteins have both enzymatic activity and specific activity for p-nitrophenyl butyrate (C4) in vitro. Moreover, the enzymatic activity of recombinant AtCXE8 was twofold higher than that of AtCXE9. To gain a better understanding of the endogenous role of the AtCXE8 gene in Arabidopsis, we identified an enhancer trap T-DNA mutant (AtCXE8_KO) and used it to show that the AtCXE8 gene was induced in response to fungal infection. AtCXE8_KO plants were also more susceptible to infections than wild-type Col-0 plants. Moreover, overexpression of the AtCXE8 gene in transgenic Arabidopsis plants led to enhanced disease resistance against B. cinerea. Taken together, our data indicate that AtCXE8 plays a role in promoting resistance to fungal invasion.

Original languageEnglish
Pages (from-to)109-119
Number of pages11
JournalPlant Biotechnology Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2013

Keywords

  • Abiotic stress
  • Botrytis cinerea
  • Carboxylesterase
  • Disease resistance

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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