Molecular characterization of the region encoding integrative functions from enterococcal bacteriophage φFC1

Minjung Kim, Jin Young Lee, Young Woo Kim, Ha Chin Sung, Hyo-Ihl Chang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bacteriophage φFC1 is a temperate phage which was identified as a prophage in the Enterococcus faecalis KBL703 chromosome. Phage φFC1 integrates into the host chromosome by site-specific recombination. The phage attachment site P (attP) was localized within the 0.65-kb XhoI-HindIII fragment and the nucleotide sequence of the region was determined. An open reading frame (mj1) which adjoined the phage attachment site encoded a deduced protein related to the site-specific recombinase family. The organization of this region was comparable to other site-specific recombination systems. The molecular weight of the expressed MJ1 in E. coli was in good agreement with the predicted 53,537 Da of the mj1 gene product. Elucidation of the phage-specific integration process in this study would provide useful genetic tools such as a chromosomal integration system.

Original languageEnglish
Pages (from-to)448-454
Number of pages7
JournalJournal of Biochemistry and Molecular Biology
Volume29
Issue number5
Publication statusPublished - 1996 Sep 30

Keywords

  • Attachment site P (attP)
  • Site-specific recombinase
  • Site-specific recombinase family
  • Site-specific recombination

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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