Molecular cloning and analysis of the aroD gene encoding 3-dehydroquinate dehydratase from Corynebacterium glutamicum

Ky Yeon Park, Jin Sook Lee, Soon Young Choi, Dae Ho Cho, Choong Ill Cheon, Eunsook Song, Kyung Hee Min, Hyunk Chung, Heung Sick Lee, Myeong Sok Lee

Research output: Contribution to journalArticle

Abstract

The aroD gene was cloned from Corynebacterium glutamicum by complementation of Escherichia coli aromatic amimo acid auxotroph. The E. coli aroD gene codes for 3-dehydroquinte dehydratase (dehydroquinase), an enzyme involved in the aromatic amino acid biosynthesis pathway. The cloned insert DNA fragments in the recombinant plasmids, named pROD5, pROD8, and pROD14, were physically mapped with several restriction endonucleases. We determined the DNA nucleotide sequences of the fragment and reported here the sequences of the aroD gene. The complete nucleotide sequences of the aroD gene showed an open reading frame encoding a protein of 145 amino acids with a deduced molecular weight of about 16 kDa. Comparison of this deduced amino acid sequences of C. glutamicum aroD gene with those of the enzyme from Pseudomonas aeruginosa, Mycobacterium tuberculosis and Actinobacillus pleuropneumonia revealed 51%, 53% and 54% similarity, respectively.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalKorean Journal of Genetics
Volume25
Issue number4
Publication statusPublished - 2003 Dec 1
Externally publishedYes

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Keywords

  • 3-dehydroquinate dehydratase
  • aroD gene
  • Aromatic amino acid
  • Corynebacterium glutamicum
  • Heterologous complementation

ASJC Scopus subject areas

  • Genetics

Cite this

Park, K. Y., Lee, J. S., Choi, S. Y., Cho, D. H., Cheon, C. I., Song, E., Min, K. H., Chung, H., Lee, H. S., & Lee, M. S. (2003). Molecular cloning and analysis of the aroD gene encoding 3-dehydroquinate dehydratase from Corynebacterium glutamicum. Korean Journal of Genetics, 25(4), 369-377.