Molecular cloning and characterization of a GH11 endoxylanase from Chaetomium globosum, and its use in enzymatic pretreatment of biomass

Raushan Kumar Singh, Manish Kumar Tiwari, Dongwook Kim, Yun Chan Kang, Priyadharshini Ramachandran, Jung Kul Lee

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

An endo-1,4-β-xylanase gene, xylcg, was cloned from Chaetomium globosum and successfully expressed in Escherichia coli. The complete gene of 675 bp was amplified, cloned into the pET 28(a) vector, and expressed. The optimal conditions for the highest activity of the purified recombinant XylCg were observed at a temperature of 40 C and pH of 5.5. Using oat-spelt xylan, the determined K m, V max, and k cat/K m values were 0.243 mg ml-1, 4,530 U mg-1 protein, and 7,640 ml s-1 mg-1, respectively. A homology model and sequence analysis of XylCg, along with the biochemical properties, confirmed that XylCg belongs to the GH11 family. Rice straw pretreated with XylCg showed 30 % higher conversion yield than the rice straw pretreated with a commercial xylanase. Although xylanases have been characterized from fungal and bacterial sources, C. globosum XylCg is distinguished from other xylanases by its high catalytic efficiency and its effectiveness in the pretreatment of lignocellulosic biomass.

Original languageEnglish
Pages (from-to)7205-7214
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume97
Issue number16
DOIs
Publication statusPublished - 2013 Aug 1
Externally publishedYes

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Keywords

  • Chaetomium globosum
  • Endo-1,4-β-xylanase
  • Homology modeling
  • Pretreatment
  • Saccharification

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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