Molecular cloning and characterization of a novel low temperature-induced gene, blti2, from barley (Hordeum vulgare L.)

Sung Chul Bahn, Min Seok Bae, Yong Bum Park, Seung Ick Oh, Ji Ung Jeung, Jung Myung Bae, Young Soo Chung, Jeong Sheop Shin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The Suppression Subtractive Hybridization (SSH) was used to isolate large numbers of low temperature-induced genes from the cold-treated winter barley (Hordeum vulgare L. cv. Dongbori). One hundred and sixty blti (barley low temperature-induced) cDNA clones were obtained. Northern blot analyses showed that several blti clones were differentially expressed by treatment of low temperature, NaCl, dehydration and ABA. One of the clones, blti2, was induced from 3 to 72 h after cold treatment while its transcript was detected only at 12 h after ABA treatment, indicating that the expression of blti2 by low temperature was regulated by an ABA-independent pathway. The full-length cDNA sequences were 944 nucleotides long and the open reading frame consisted of 492 nucleotides encoding 164 amino acids. Nucleotide sequences showed no sequence homology with the previously reported low temperature-responsive (LTR) barley genes, and the deduced amino acid sequences revealed that the blti2 contains three membrane-spanning regions. These results suggest that blti2 is a novel transmembrane protein induced by low temperature.

Original languageEnglish
Pages (from-to)134-137
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1522
Issue number2
DOIs
Publication statusPublished - 2001 Dec 3

Keywords

  • Barley (Hordeum vulgare L. cv. Dongbori)
  • Differential gene expression
  • Low temperature induction
  • Suppression subtractive hybridization
  • blti2

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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