Molecular cloning and characterization of a novel phospholipase C, PLC-η

Jong-Ik Hwang, Yong Seok Oh, Kum Joo Shin, Hyun Kim, Sung Ho Ryu, Pann Ghill Suh

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

PLC (phospholipase C) plays an important role in intracellular signal transduction by hydrolysing phosphatidylinositol 4,5-bisphosphate, a membrane phospholipid. To date, 12 members of the mammalian PLC isoforms have been identified and classified into five isotypes β, γ, δ, ε and ζ, which are regulated by distinct mechanisms. In the present study, we describe the identification of a novel PLC isoform in the brains of human and mouse, named PLC-η, which contains the conserved pleckstrin homology domain, X and Y domains for catalytic activity and the C2 domain. The first identified gene encoded 1002 (human) or 1003 (mouse) amino acids with an estimated molecular mass of 115 kDa. The purified recombinant PLC-η exhibited Ca 2+-dependent catalytic activity on phosphatidylinositol 4,5-bisphosphate. Furthermore, molecular biological analysis revealed that the PLC-η gene was transcribed to several splicing variants. Although some transcripts were detected in most of the tissues we examined, the transcript encoding 115 kDa was restricted to the brain and lung. In addition, the expression of the 115 kDa protein was defined in only nerve tissues such as the brain and spinal cord. In situ hybridization analysis with brain revealed that PLC-η was abundantly expressed in various regions including cerebral cortex, hippocampus, zona incerta and cerebellar Purkinje cell layer, which are neuronal cell-enriched regions. These results suggest that PLC-η may perform fundamental roles in the brain.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalBiochemical Journal
Volume389
Issue number1
DOIs
Publication statusPublished - 2005 Jul 1

Fingerprint

Cloning
Molecular Cloning
Type C Phospholipases
Brain
Phosphatidylinositols
Catalyst activity
Protein Isoforms
Genes
Tissue
Nerve Tissue
Signal transduction
Purkinje Cells
Molecular mass
Cerebral Cortex
In Situ Hybridization
Signal Transduction
Hippocampus
Catalytic Domain
Phospholipids
Spinal Cord

Keywords

  • Ca
  • Inositol 1,4,5-trisphosphate
  • Molecular cloning
  • Phosphatidylinositol 4,5-bisphosphate
  • Phospholipase
  • PLC-η

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular cloning and characterization of a novel phospholipase C, PLC-η. / Hwang, Jong-Ik; Oh, Yong Seok; Shin, Kum Joo; Kim, Hyun; Ryu, Sung Ho; Suh, Pann Ghill.

In: Biochemical Journal, Vol. 389, No. 1, 01.07.2005, p. 181-186.

Research output: Contribution to journalArticle

Hwang, Jong-Ik ; Oh, Yong Seok ; Shin, Kum Joo ; Kim, Hyun ; Ryu, Sung Ho ; Suh, Pann Ghill. / Molecular cloning and characterization of a novel phospholipase C, PLC-η. In: Biochemical Journal. 2005 ; Vol. 389, No. 1. pp. 181-186.
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