Molecular cloning and characterization of alternatively spliced transcripts of the turkey pituitary adenylate cyclase-activating polypeptide

Seung Jun Yoo, Seungkwon You, Hyunggee Kim, Sung Chan Kim, Yun Jaie Choi, Mohamed El Halawani, James Farris, Douglas N. Foster

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) increases the release of growth hormone (GH) and prolactin (PRL) in mammals. However, the evolutionary and functional relationships of PACAP, GH, and PRL are not clear. To understand how PACAP is regulated in the turkey, a turkey PACAP (tPACAP) cDNA has been cloned by the combination of reverse transcription-polymerase chain reaction and the rapid amplification of cDNA 5′- and 3′-ends. The deduced amino acid sequence of tPACAP-38 and turkey PACAP-related peptide (tPRP) displayed 87-97 and 52-63% similarity when compared to a variety of known PACAP-38 and PRP sequences, respectively. Two major transcripts (1.3 and 3.0 kb) of tPACAP were detected by Northern blot analysis. The highest levels of tPACAP mRNA were shown to be expressed in the hypothalamus, the cerebellum, and the cerebrum. In contrast, most of the other tissues tested expressed relatively low steady-state levels of tPACAP mRNA. Alternative splicing of tPACAP resulted in the expression of two different isoforms. The smaller form of tPACAP was expressed in the hypothalamus during early embryonic development and decreased significantly in later stages.

Original languageEnglish
Pages (from-to)326-335
Number of pages10
JournalGeneral and Comparative Endocrinology
Volume120
Issue number3
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • Adenylate cyclase
  • Exon skipping
  • Hypothalamus
  • Neurotransmitter
  • PACAP
  • Turkey
  • VIP
  • tGH
  • tPACAP
  • tPRL
  • tPRP

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

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