Molecular cloning and characterization of alternatively spliced transcripts of the turkey pituitary adenylate cyclase-activating polypeptide

Seung Jun Yoo, Seungkwon You, Hyunggee Kim, Sung Chan Kim, Yun Jaie Choi, Mohamed El Halawani, James Farris, Douglas N. Foster

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) increases the release of growth hormone (GH) and prolactin (PRL) in mammals. However, the evolutionary and functional relationships of PACAP, GH, and PRL are not clear. To understand how PACAP is regulated in the turkey, a turkey PACAP (tPACAP) cDNA has been cloned by the combination of reverse transcription-polymerase chain reaction and the rapid amplification of cDNA 5′- and 3′-ends. The deduced amino acid sequence of tPACAP-38 and turkey PACAP-related peptide (tPRP) displayed 87-97 and 52-63% similarity when compared to a variety of known PACAP-38 and PRP sequences, respectively. Two major transcripts (1.3 and 3.0 kb) of tPACAP were detected by Northern blot analysis. The highest levels of tPACAP mRNA were shown to be expressed in the hypothalamus, the cerebellum, and the cerebrum. In contrast, most of the other tissues tested expressed relatively low steady-state levels of tPACAP mRNA. Alternative splicing of tPACAP resulted in the expression of two different isoforms. The smaller form of tPACAP was expressed in the hypothalamus during early embryonic development and decreased significantly in later stages.

Original languageEnglish
Pages (from-to)326-335
Number of pages10
JournalGeneral and Comparative Endocrinology
Volume120
Issue number3
DOIs
Publication statusPublished - 2000 Dec 1
Externally publishedYes

Fingerprint

Pituitary Adenylate Cyclase-Activating Polypeptide
Molecular Cloning
Prolactin
Growth Hormone
Hypothalamus
Complementary DNA
Messenger RNA
Peptide Hormones
Alternative Splicing
Cerebrum
Northern Blotting
Cerebellum
Reverse Transcription
Embryonic Development
Mammals
Amino Acid Sequence
Protein Isoforms

Keywords

  • Adenylate cyclase
  • Exon skipping
  • Hypothalamus
  • Neurotransmitter
  • PACAP
  • tGH
  • tPACAP
  • tPRL
  • tPRP
  • Turkey
  • VIP

ASJC Scopus subject areas

  • Endocrinology

Cite this

Molecular cloning and characterization of alternatively spliced transcripts of the turkey pituitary adenylate cyclase-activating polypeptide. / Yoo, Seung Jun; You, Seungkwon; Kim, Hyunggee; Kim, Sung Chan; Choi, Yun Jaie; El Halawani, Mohamed; Farris, James; Foster, Douglas N.

In: General and Comparative Endocrinology, Vol. 120, No. 3, 01.12.2000, p. 326-335.

Research output: Contribution to journalArticle

Yoo, Seung Jun ; You, Seungkwon ; Kim, Hyunggee ; Kim, Sung Chan ; Choi, Yun Jaie ; El Halawani, Mohamed ; Farris, James ; Foster, Douglas N. / Molecular cloning and characterization of alternatively spliced transcripts of the turkey pituitary adenylate cyclase-activating polypeptide. In: General and Comparative Endocrinology. 2000 ; Vol. 120, No. 3. pp. 326-335.
@article{e5a7373c10aa473c936144f5c89ba030,
title = "Molecular cloning and characterization of alternatively spliced transcripts of the turkey pituitary adenylate cyclase-activating polypeptide",
abstract = "Pituitary adenylate cyclase-activating polypeptide (PACAP) increases the release of growth hormone (GH) and prolactin (PRL) in mammals. However, the evolutionary and functional relationships of PACAP, GH, and PRL are not clear. To understand how PACAP is regulated in the turkey, a turkey PACAP (tPACAP) cDNA has been cloned by the combination of reverse transcription-polymerase chain reaction and the rapid amplification of cDNA 5′- and 3′-ends. The deduced amino acid sequence of tPACAP-38 and turkey PACAP-related peptide (tPRP) displayed 87-97 and 52-63{\%} similarity when compared to a variety of known PACAP-38 and PRP sequences, respectively. Two major transcripts (1.3 and 3.0 kb) of tPACAP were detected by Northern blot analysis. The highest levels of tPACAP mRNA were shown to be expressed in the hypothalamus, the cerebellum, and the cerebrum. In contrast, most of the other tissues tested expressed relatively low steady-state levels of tPACAP mRNA. Alternative splicing of tPACAP resulted in the expression of two different isoforms. The smaller form of tPACAP was expressed in the hypothalamus during early embryonic development and decreased significantly in later stages.",
keywords = "Adenylate cyclase, Exon skipping, Hypothalamus, Neurotransmitter, PACAP, tGH, tPACAP, tPRL, tPRP, Turkey, VIP",
author = "Yoo, {Seung Jun} and Seungkwon You and Hyunggee Kim and Kim, {Sung Chan} and Choi, {Yun Jaie} and {El Halawani}, Mohamed and James Farris and Foster, {Douglas N.}",
year = "2000",
month = "12",
day = "1",
doi = "10.1006/gcen.2000.7567",
language = "English",
volume = "120",
pages = "326--335",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Molecular cloning and characterization of alternatively spliced transcripts of the turkey pituitary adenylate cyclase-activating polypeptide

AU - Yoo, Seung Jun

AU - You, Seungkwon

AU - Kim, Hyunggee

AU - Kim, Sung Chan

AU - Choi, Yun Jaie

AU - El Halawani, Mohamed

AU - Farris, James

AU - Foster, Douglas N.

PY - 2000/12/1

Y1 - 2000/12/1

N2 - Pituitary adenylate cyclase-activating polypeptide (PACAP) increases the release of growth hormone (GH) and prolactin (PRL) in mammals. However, the evolutionary and functional relationships of PACAP, GH, and PRL are not clear. To understand how PACAP is regulated in the turkey, a turkey PACAP (tPACAP) cDNA has been cloned by the combination of reverse transcription-polymerase chain reaction and the rapid amplification of cDNA 5′- and 3′-ends. The deduced amino acid sequence of tPACAP-38 and turkey PACAP-related peptide (tPRP) displayed 87-97 and 52-63% similarity when compared to a variety of known PACAP-38 and PRP sequences, respectively. Two major transcripts (1.3 and 3.0 kb) of tPACAP were detected by Northern blot analysis. The highest levels of tPACAP mRNA were shown to be expressed in the hypothalamus, the cerebellum, and the cerebrum. In contrast, most of the other tissues tested expressed relatively low steady-state levels of tPACAP mRNA. Alternative splicing of tPACAP resulted in the expression of two different isoforms. The smaller form of tPACAP was expressed in the hypothalamus during early embryonic development and decreased significantly in later stages.

AB - Pituitary adenylate cyclase-activating polypeptide (PACAP) increases the release of growth hormone (GH) and prolactin (PRL) in mammals. However, the evolutionary and functional relationships of PACAP, GH, and PRL are not clear. To understand how PACAP is regulated in the turkey, a turkey PACAP (tPACAP) cDNA has been cloned by the combination of reverse transcription-polymerase chain reaction and the rapid amplification of cDNA 5′- and 3′-ends. The deduced amino acid sequence of tPACAP-38 and turkey PACAP-related peptide (tPRP) displayed 87-97 and 52-63% similarity when compared to a variety of known PACAP-38 and PRP sequences, respectively. Two major transcripts (1.3 and 3.0 kb) of tPACAP were detected by Northern blot analysis. The highest levels of tPACAP mRNA were shown to be expressed in the hypothalamus, the cerebellum, and the cerebrum. In contrast, most of the other tissues tested expressed relatively low steady-state levels of tPACAP mRNA. Alternative splicing of tPACAP resulted in the expression of two different isoforms. The smaller form of tPACAP was expressed in the hypothalamus during early embryonic development and decreased significantly in later stages.

KW - Adenylate cyclase

KW - Exon skipping

KW - Hypothalamus

KW - Neurotransmitter

KW - PACAP

KW - tGH

KW - tPACAP

KW - tPRL

KW - tPRP

KW - Turkey

KW - VIP

UR - http://www.scopus.com/inward/record.url?scp=0034517752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034517752&partnerID=8YFLogxK

U2 - 10.1006/gcen.2000.7567

DO - 10.1006/gcen.2000.7567

M3 - Article

C2 - 11121297

AN - SCOPUS:0034517752

VL - 120

SP - 326

EP - 335

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 3

ER -