Molecular cloning and characterization of the germ cell-related nuclear orphan receptor in chickens

Sang In Lee, Jin Kyoo Kim, Hyun Jeong Park, Hyun Jun Jang, Hyung Chul Lee, Taesun Min, Gwonhwa Song, Jae Yong Han

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Nuclear receptor subfamily 6, group A, member 1 (NR6A1), also known as germ cell nuclear factor/retinoid receptor-related testis-associated receptor and neuronal cell nuclear factor, is a member of the nuclear orphan receptor superfamily. NR6A1 has been cloned in various species including humans and mice, but it has been scarcely investigated in avian species. In the present study, we cloned the chicken NR6A1 (cNR6A1) from a testis cDNA library. The cloned cNR6A1 sequence was mapped to chromosome 17 and contained an open reading frame of 1.4 kb encoding 445 amino acids. Multiple alignment analysis of the cNR6A1 protein-coding sequence with NR6A1s from humans, mice, boars, rats, zebrafish, and Xenopus showed high degrees of homology, 89%, 90%, 89%, 88%, 83%, and 87%, respectively. Using RNA interference, changes in the expression of pluripotency-, germ cell-, and differentiation-related key genes by silencing of cNR6A1 were validated in chicken blastodermderived embryonic stem cells. Among those genes, the relative expression levels of POU5F1, CRIPTO, DAZL, DDX4, BMP15, GSC, and SOX7 changed significantly compared to the control group. We also confirmed that the activity of alkaline phosphatase, known as a pluripotency marker, was maintained by cNR6A1 gene silencing in chicken blastodermal cells. Collectively, our data suggest that cNR6A1 may play an important role during chicken embryonic development and differentiation.

Original languageEnglish
Pages (from-to)273-284
Number of pages12
JournalMolecular Reproduction and Development
Volume77
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1
Externally publishedYes

Fingerprint

Orphan Nuclear Receptors
Nuclear Receptor Subfamily 6, Group A, Member 1
Molecular Cloning
Germ Cells
Chickens
Gene Silencing
Chromosomes, Human, Pair 17
Zebrafish
Embryonic Stem Cells
Xenopus
RNA Interference
Gene Library
Open Reading Frames
Embryonic Development
Alkaline Phosphatase
Testis
Cell Differentiation
Gene Expression
Amino Acids

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Lee, S. I., Kim, J. K., Park, H. J., Jang, H. J., Lee, H. C., Min, T., ... Han, J. Y. (2010). Molecular cloning and characterization of the germ cell-related nuclear orphan receptor in chickens. Molecular Reproduction and Development, 77(3), 273-284. https://doi.org/10.1002/mrd.21144

Molecular cloning and characterization of the germ cell-related nuclear orphan receptor in chickens. / Lee, Sang In; Kim, Jin Kyoo; Park, Hyun Jeong; Jang, Hyun Jun; Lee, Hyung Chul; Min, Taesun; Song, Gwonhwa; Han, Jae Yong.

In: Molecular Reproduction and Development, Vol. 77, No. 3, 01.03.2010, p. 273-284.

Research output: Contribution to journalArticle

Lee, Sang In ; Kim, Jin Kyoo ; Park, Hyun Jeong ; Jang, Hyun Jun ; Lee, Hyung Chul ; Min, Taesun ; Song, Gwonhwa ; Han, Jae Yong. / Molecular cloning and characterization of the germ cell-related nuclear orphan receptor in chickens. In: Molecular Reproduction and Development. 2010 ; Vol. 77, No. 3. pp. 273-284.
@article{8405a0702e8a456698ae8b3f8090df62,
title = "Molecular cloning and characterization of the germ cell-related nuclear orphan receptor in chickens",
abstract = "Nuclear receptor subfamily 6, group A, member 1 (NR6A1), also known as germ cell nuclear factor/retinoid receptor-related testis-associated receptor and neuronal cell nuclear factor, is a member of the nuclear orphan receptor superfamily. NR6A1 has been cloned in various species including humans and mice, but it has been scarcely investigated in avian species. In the present study, we cloned the chicken NR6A1 (cNR6A1) from a testis cDNA library. The cloned cNR6A1 sequence was mapped to chromosome 17 and contained an open reading frame of 1.4 kb encoding 445 amino acids. Multiple alignment analysis of the cNR6A1 protein-coding sequence with NR6A1s from humans, mice, boars, rats, zebrafish, and Xenopus showed high degrees of homology, 89{\%}, 90{\%}, 89{\%}, 88{\%}, 83{\%}, and 87{\%}, respectively. Using RNA interference, changes in the expression of pluripotency-, germ cell-, and differentiation-related key genes by silencing of cNR6A1 were validated in chicken blastodermderived embryonic stem cells. Among those genes, the relative expression levels of POU5F1, CRIPTO, DAZL, DDX4, BMP15, GSC, and SOX7 changed significantly compared to the control group. We also confirmed that the activity of alkaline phosphatase, known as a pluripotency marker, was maintained by cNR6A1 gene silencing in chicken blastodermal cells. Collectively, our data suggest that cNR6A1 may play an important role during chicken embryonic development and differentiation.",
author = "Lee, {Sang In} and Kim, {Jin Kyoo} and Park, {Hyun Jeong} and Jang, {Hyun Jun} and Lee, {Hyung Chul} and Taesun Min and Gwonhwa Song and Han, {Jae Yong}",
year = "2010",
month = "3",
day = "1",
doi = "10.1002/mrd.21144",
language = "English",
volume = "77",
pages = "273--284",
journal = "Molecular Reproduction and Development",
issn = "1040-452X",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Molecular cloning and characterization of the germ cell-related nuclear orphan receptor in chickens

AU - Lee, Sang In

AU - Kim, Jin Kyoo

AU - Park, Hyun Jeong

AU - Jang, Hyun Jun

AU - Lee, Hyung Chul

AU - Min, Taesun

AU - Song, Gwonhwa

AU - Han, Jae Yong

PY - 2010/3/1

Y1 - 2010/3/1

N2 - Nuclear receptor subfamily 6, group A, member 1 (NR6A1), also known as germ cell nuclear factor/retinoid receptor-related testis-associated receptor and neuronal cell nuclear factor, is a member of the nuclear orphan receptor superfamily. NR6A1 has been cloned in various species including humans and mice, but it has been scarcely investigated in avian species. In the present study, we cloned the chicken NR6A1 (cNR6A1) from a testis cDNA library. The cloned cNR6A1 sequence was mapped to chromosome 17 and contained an open reading frame of 1.4 kb encoding 445 amino acids. Multiple alignment analysis of the cNR6A1 protein-coding sequence with NR6A1s from humans, mice, boars, rats, zebrafish, and Xenopus showed high degrees of homology, 89%, 90%, 89%, 88%, 83%, and 87%, respectively. Using RNA interference, changes in the expression of pluripotency-, germ cell-, and differentiation-related key genes by silencing of cNR6A1 were validated in chicken blastodermderived embryonic stem cells. Among those genes, the relative expression levels of POU5F1, CRIPTO, DAZL, DDX4, BMP15, GSC, and SOX7 changed significantly compared to the control group. We also confirmed that the activity of alkaline phosphatase, known as a pluripotency marker, was maintained by cNR6A1 gene silencing in chicken blastodermal cells. Collectively, our data suggest that cNR6A1 may play an important role during chicken embryonic development and differentiation.

AB - Nuclear receptor subfamily 6, group A, member 1 (NR6A1), also known as germ cell nuclear factor/retinoid receptor-related testis-associated receptor and neuronal cell nuclear factor, is a member of the nuclear orphan receptor superfamily. NR6A1 has been cloned in various species including humans and mice, but it has been scarcely investigated in avian species. In the present study, we cloned the chicken NR6A1 (cNR6A1) from a testis cDNA library. The cloned cNR6A1 sequence was mapped to chromosome 17 and contained an open reading frame of 1.4 kb encoding 445 amino acids. Multiple alignment analysis of the cNR6A1 protein-coding sequence with NR6A1s from humans, mice, boars, rats, zebrafish, and Xenopus showed high degrees of homology, 89%, 90%, 89%, 88%, 83%, and 87%, respectively. Using RNA interference, changes in the expression of pluripotency-, germ cell-, and differentiation-related key genes by silencing of cNR6A1 were validated in chicken blastodermderived embryonic stem cells. Among those genes, the relative expression levels of POU5F1, CRIPTO, DAZL, DDX4, BMP15, GSC, and SOX7 changed significantly compared to the control group. We also confirmed that the activity of alkaline phosphatase, known as a pluripotency marker, was maintained by cNR6A1 gene silencing in chicken blastodermal cells. Collectively, our data suggest that cNR6A1 may play an important role during chicken embryonic development and differentiation.

UR - http://www.scopus.com/inward/record.url?scp=76649123092&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76649123092&partnerID=8YFLogxK

U2 - 10.1002/mrd.21144

DO - 10.1002/mrd.21144

M3 - Article

C2 - 20020523

AN - SCOPUS:76649123092

VL - 77

SP - 273

EP - 284

JO - Molecular Reproduction and Development

JF - Molecular Reproduction and Development

SN - 1040-452X

IS - 3

ER -