Molecular cloning and comparative analysis of immunoglobulin heavy chain genes from Phasianus colchicus, Meleagris gallopavo, and Coturnix japonica

Jin Won Choi, Jin Kyoo Kim, Hee Won Seo, Byung Wook Cho, Gwonhwa Song, Jae Yong Han

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

To date, immunoglobulin (Ig) genes have only been fully characterized in a small number of aves, which pose a major obstacle to understanding Ig evolution. Thus, we cloned the cDNAs of three immunoglobulin classes, IgA, IgM, and IgY, from Phasianus colchicus, Coturnix japonica, and Meleagris gallopavo. Multiple sequence alignments revealed that the highest degree of sequence homology in all Ig classes was observed between pheasant and turkey whereas the degree of homology between the galliforms and non-galliforms was relatively low compared to that among the galliforms. When the constant region domains of the four human Ig classes were compared with the corresponding regions in aves, the average percent homology between human CH2 and avian CH3, and between human CH3 and avian CH4, was greater than between identical domains in IgA and IgY, which are in partial agreement with the hypothesis that the avian CH2 domain evolved to form the mammalian hinge via domain condensation. Phylogenetic analysis showed that the galliform Ig heavy chain constant regions were divided into quail and the common ancestor of chicken, turkey, and pheasant, and that chicken was separated from turkey and pheasant, which were grouped together. These results add to our knowledge of galliform Igs and the diversification of these genes.

Original languageEnglish
Pages (from-to)248-256
Number of pages9
JournalVeterinary Immunology and Immunopathology
Volume136
Issue number3-4
DOIs
Publication statusPublished - 2010 Aug 15
Externally publishedYes

Keywords

  • Immunoglobulin heavy chain
  • Pheasant
  • Quail
  • Turkey

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

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