Molecular cloning and expression analysis of CD127 in pig

Yu Jin Hur, Jong Gun Kim, Kwang Youn Whang, Young Bong Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

CD127 (the α chain of the IL-7 receptor) is an 80kDa type I membrane glycoprotein that mediates the signal for the differentiation, maturation, and survival of lymphocyte development. In this study, the full-length cDNA of pig Cd127 was cloned and sequenced. Pig CD127 cDNA contains an open reading frame (1,380bp) encoding 459 amino acids. Phylogenetic analysis revealed that pig CD127 is most homologous to that of cow (Bos taurus) with 87% amino acid identity and less homologous to that of chicken (Gallus gallus) with 36% amino acid identity among those of species. Structurally, pig CD127 has well-conserved cytoplasmic tail that transmits a signal and little diversity of extracellular domain, compared with those of other mammalian species. Expression analysis indicates that the mRNA transcript of pig CD127 is mainly detected in lymphoid tissues, but still detected in nonlymphoid tissues. Future studies will be focused on determining a more defined expression pattern of pig CD127 and the role of CD127 during the lymphocyte development in pigs.

Original languageEnglish
Pages (from-to)40-45
Number of pages6
JournalAnimal Biotechnology
Volume20
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

Keywords

  • CD127 (IL-7 receptor chain)
  • Cytokine resceptor
  • Development
  • Immune response
  • Pig

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Animal Science and Zoology

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