Molecular cloning and expression analysis of pig CD138

Joonbeom Bae, Seonah Jeong, Ju Yeon Lee, Hyun Jeong Lee, Bong Hwan Choi, Ji Eun Kim, Inho Choi, Taehoon Chun

Research output: Contribution to journalArticle

Abstract

CD138 (syndecan-1) interacts with various components of the extracellular matrix and associates with the actin cytoskeleton. In this study, we cloned pig CD138 cDNA and determined its complete cDNA sequence. Pig CD138 cDNA contained an open reading frame (930. bp) encoding 309 amino acids with five well conserved putative glycosaminoglycan attachment sites, a putative cleavage site for matrix metalloproteinases, and conserved motifs involved in signal transduction among mammalian species. Pig CD138 mRNA was detected in various tissues, including lymphoid and non-lymphoid organs, indicating the multicellular functions of CD138 in pigs. Western blot and flow cytometry analyses detected an approximate 35. kDa pig CD138 protein expressed on the cell surface. Further immunohistochemistry analysis revealed that CD138 expression was mainly observed in submucosa and lamina propria of the pig small intestine. Further study will be necessary to define the functional importance of CD138 during specific infectious diseases in pigs.

Original languageEnglish
Pages (from-to)1021-1025
Number of pages5
JournalResearch in Veterinary Science
Volume95
Issue number3
DOIs
Publication statusPublished - 2013 Dec

Keywords

  • CD138
  • CDNA cloning
  • Expression
  • Pig
  • Syndecan

ASJC Scopus subject areas

  • veterinary(all)

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