Molecular cloning and expression analysis of pig CD7

Ju Yeon Lee, Joonbeom Bae, Inho Choi, Chung Gyu Park, Taehoon Chun

Research output: Contribution to journalArticle

Abstract

CD7 is an integral membrane protein which mediates an important signal to mediate the differentiation, activation, and regulation of some T cells and NK cells. However, only human and mouse CD7 have been identified and studied among mammalian species. In this study, we cloned pig CD7 cDNA and determined its complete cDNA sequence. Pig CD7 cDNA contained an open reading frame (627 bp) encoding 208 amino acids with well conserved motifs involved in signal transduction within cytoplasmic tail among mammalian species. Pig CD7 mRNA was detected by RT-PCR in mainly lymphoid tissues, indicating the conserved functions of CD7 in pigs. Moreover, we generated soluble pig CD7 fusion immunoglobulin (pig CD7Ig) containing extracellular domain of pig CD7 to test whether pig CD7 binds to pig galectin-3. Flow cytometry and immunohistochemistry analyses indicated that soluble pig CD7Ig can bind to galectin-3 expressed in macrophages and epithelial cells of small intestine. These results help to analyze the structural relationship between CD7 and its ligand transferring signal transduction among mammalian species.

Original languageEnglish
Pages (from-to)257-263
Number of pages7
JournalVeterinary Research Communications
Volume38
Issue number3
DOIs
Publication statusPublished - 2014 Jan 1

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Molecular Cloning
molecular cloning
Swine
swine
Galectin 3
Complementary DNA
immunoglobulins
signal transduction
Immunoglobulins
Signal Transduction
natural killer cells
Lymphoid Tissue
membrane proteins
Natural Killer Cells
Open Reading Frames
Small Intestine
immunohistochemistry
flow cytometry
open reading frames
small intestine

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Molecular cloning and expression analysis of pig CD7. / Lee, Ju Yeon; Bae, Joonbeom; Choi, Inho; Park, Chung Gyu; Chun, Taehoon.

In: Veterinary Research Communications, Vol. 38, No. 3, 01.01.2014, p. 257-263.

Research output: Contribution to journalArticle

Lee, Ju Yeon ; Bae, Joonbeom ; Choi, Inho ; Park, Chung Gyu ; Chun, Taehoon. / Molecular cloning and expression analysis of pig CD7. In: Veterinary Research Communications. 2014 ; Vol. 38, No. 3. pp. 257-263.
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