Abstract
Lymphocyte activation gene-3 (LAG-3; CD223) is a co-receptor that may function as a down-modulator of T cell activity via an interaction with MHC class II molecules. In this study, we cloned and sequenced the complete cDNA sequence of pig Lag3. Pig Lag3 cDNA contains an open reading frame (1524 bp) encoding 507 amino acids. The putative amino acid identity of pig LAG-3 with those of the human, rat, and mouse is 78%, 66%, and 67%, respectively. Pig Lag3 mRNA transcript was mainly expressed in lymphoid tissues, and was inducible upon stimulation of phytohemagglutinin (PHA). Next, we developed mouse antiserum against the extracellular domain (D1 and D2 domains) of pig LAG-3. Pig LAG-3 is detected as an approximately 67 kDa of a single species by Western blot analysis. Moreover, flow cytometry analysis confirmed that LAG-3 on pig T cells (CD3+ cells) is inducible upon PHA stimulation. These results indicated that the structure and expression pattern of pig LAG-3 are conserved among mammalian species.
| Original language | English |
|---|---|
| Pages (from-to) | 72-79 |
| Number of pages | 8 |
| Journal | Veterinary Immunology and Immunopathology |
| Volume | 133 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2010 Jan 15 |
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Keywords
- Co-receptor
- Immune homeostasis
- Lymphocyte activation gene-3
- Pig
- T cell activation
ASJC Scopus subject areas
- Immunology
- veterinary(all)
Cite this
Molecular cloning and expression analysis of pig lymphocyte activation gene-3 (LAG-3; CD223). / Kim, Seon Soo; Kim, Sang Hoon; Kang, Hyung Sik; Chung, Hee Yong; Choi, Inho; Cheon, Yong Pil; Lee, Ki Ho; Lee, Dong Mok; Park, Jongsun; Lee, Sang Yeol; Chun, Taehoon.
In: Veterinary Immunology and Immunopathology, Vol. 133, No. 1, 15.01.2010, p. 72-79.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning and expression analysis of pig lymphocyte activation gene-3 (LAG-3; CD223)
AU - Kim, Seon Soo
AU - Kim, Sang Hoon
AU - Kang, Hyung Sik
AU - Chung, Hee Yong
AU - Choi, Inho
AU - Cheon, Yong Pil
AU - Lee, Ki Ho
AU - Lee, Dong Mok
AU - Park, Jongsun
AU - Lee, Sang Yeol
AU - Chun, Taehoon
PY - 2010/1/15
Y1 - 2010/1/15
N2 - Lymphocyte activation gene-3 (LAG-3; CD223) is a co-receptor that may function as a down-modulator of T cell activity via an interaction with MHC class II molecules. In this study, we cloned and sequenced the complete cDNA sequence of pig Lag3. Pig Lag3 cDNA contains an open reading frame (1524 bp) encoding 507 amino acids. The putative amino acid identity of pig LAG-3 with those of the human, rat, and mouse is 78%, 66%, and 67%, respectively. Pig Lag3 mRNA transcript was mainly expressed in lymphoid tissues, and was inducible upon stimulation of phytohemagglutinin (PHA). Next, we developed mouse antiserum against the extracellular domain (D1 and D2 domains) of pig LAG-3. Pig LAG-3 is detected as an approximately 67 kDa of a single species by Western blot analysis. Moreover, flow cytometry analysis confirmed that LAG-3 on pig T cells (CD3+ cells) is inducible upon PHA stimulation. These results indicated that the structure and expression pattern of pig LAG-3 are conserved among mammalian species.
AB - Lymphocyte activation gene-3 (LAG-3; CD223) is a co-receptor that may function as a down-modulator of T cell activity via an interaction with MHC class II molecules. In this study, we cloned and sequenced the complete cDNA sequence of pig Lag3. Pig Lag3 cDNA contains an open reading frame (1524 bp) encoding 507 amino acids. The putative amino acid identity of pig LAG-3 with those of the human, rat, and mouse is 78%, 66%, and 67%, respectively. Pig Lag3 mRNA transcript was mainly expressed in lymphoid tissues, and was inducible upon stimulation of phytohemagglutinin (PHA). Next, we developed mouse antiserum against the extracellular domain (D1 and D2 domains) of pig LAG-3. Pig LAG-3 is detected as an approximately 67 kDa of a single species by Western blot analysis. Moreover, flow cytometry analysis confirmed that LAG-3 on pig T cells (CD3+ cells) is inducible upon PHA stimulation. These results indicated that the structure and expression pattern of pig LAG-3 are conserved among mammalian species.
KW - Co-receptor
KW - Immune homeostasis
KW - Lymphocyte activation gene-3
KW - Pig
KW - T cell activation
UR - http://www.scopus.com/inward/record.url?scp=71949122179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=71949122179&partnerID=8YFLogxK
U2 - 10.1016/j.vetimm.2009.07.001
DO - 10.1016/j.vetimm.2009.07.001
M3 - Article
C2 - 19631993
AN - SCOPUS:71949122179
VL - 133
SP - 72
EP - 79
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
SN - 0165-2427
IS - 1
ER -