Molecular cloning and expression analysis of pig lymphocyte activation gene-3 (LAG-3; CD223)

Seon Soo Kim; Sang Hoon Kim; Hyung Sik Kang; Hee Yong Chung; Inho Choi; Yong Pil Cheon; Ki Ho Lee; Dong Mok Lee; Jongsun Park; Sang Yeol Lee; Taehoon Chun

doi:10.1016/j.vetimm.2009.07.001

Molecular cloning and expression analysis of pig lymphocyte activation gene-3 (LAG-3; CD223)

Seon Soo Kim, Sang Hoon Kim, Hyung Sik Kang, Hee Yong Chung, Inho Choi, Yong Pil Cheon, Ki Ho Lee, Dong Mok Lee, Jongsun Park, Sang Yeol Lee, Taehoon Chun

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Lymphocyte activation gene-3 (LAG-3; CD223) is a co-receptor that may function as a down-modulator of T cell activity via an interaction with MHC class II molecules. In this study, we cloned and sequenced the complete cDNA sequence of pig Lag3. Pig Lag3 cDNA contains an open reading frame (1524 bp) encoding 507 amino acids. The putative amino acid identity of pig LAG-3 with those of the human, rat, and mouse is 78%, 66%, and 67%, respectively. Pig Lag3 mRNA transcript was mainly expressed in lymphoid tissues, and was inducible upon stimulation of phytohemagglutinin (PHA). Next, we developed mouse antiserum against the extracellular domain (D1 and D2 domains) of pig LAG-3. Pig LAG-3 is detected as an approximately 67 kDa of a single species by Western blot analysis. Moreover, flow cytometry analysis confirmed that LAG-3 on pig T cells (CD3⁺ cells) is inducible upon PHA stimulation. These results indicated that the structure and expression pattern of pig LAG-3 are conserved among mammalian species.

Original language	English
Pages (from-to)	72-79
Number of pages	8
Journal	Veterinary Immunology and Immunopathology
Volume	133
Issue number	1
DOIs	https://doi.org/10.1016/j.vetimm.2009.07.001
Publication status	Published - 2010 Jan 15

Keywords

Co-receptor
Immune homeostasis
Lymphocyte activation gene-3
Pig
T cell activation

ASJC Scopus subject areas

Immunology
veterinary(all)

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10.1016/j.vetimm.2009.07.001

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title = "Molecular cloning and expression analysis of pig lymphocyte activation gene-3 (LAG-3; CD223)",

abstract = "Lymphocyte activation gene-3 (LAG-3; CD223) is a co-receptor that may function as a down-modulator of T cell activity via an interaction with MHC class II molecules. In this study, we cloned and sequenced the complete cDNA sequence of pig Lag3. Pig Lag3 cDNA contains an open reading frame (1524 bp) encoding 507 amino acids. The putative amino acid identity of pig LAG-3 with those of the human, rat, and mouse is 78%, 66%, and 67%, respectively. Pig Lag3 mRNA transcript was mainly expressed in lymphoid tissues, and was inducible upon stimulation of phytohemagglutinin (PHA). Next, we developed mouse antiserum against the extracellular domain (D1 and D2 domains) of pig LAG-3. Pig LAG-3 is detected as an approximately 67 kDa of a single species by Western blot analysis. Moreover, flow cytometry analysis confirmed that LAG-3 on pig T cells (CD3+ cells) is inducible upon PHA stimulation. These results indicated that the structure and expression pattern of pig LAG-3 are conserved among mammalian species.",

keywords = "Co-receptor, Immune homeostasis, Lymphocyte activation gene-3, Pig, T cell activation",

author = "Kim, {Seon Soo} and Kim, {Sang Hoon} and Kang, {Hyung Sik} and Chung, {Hee Yong} and Inho Choi and Cheon, {Yong Pil} and Lee, {Ki Ho} and Lee, {Dong Mok} and Jongsun Park and Lee, {Sang Yeol} and Taehoon Chun",

note = "Funding Information: This study was supported by Technology Development Program for Agriculture and Forestry (project number: 108089-3), Ministry for Agriculture, Forestry and Fisheries, Republic of Korea (to T. Chun) and a grant from Biogreen 21 project (project number: 20070401-034-026-007-06), Rural Development Administration, Republic of Korea (to H.-S. Kang).",

year = "2010",

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doi = "10.1016/j.vetimm.2009.07.001",

language = "English",

volume = "133",

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T1 - Molecular cloning and expression analysis of pig lymphocyte activation gene-3 (LAG-3; CD223)

AU - Kim, Seon Soo

AU - Kim, Sang Hoon

AU - Kang, Hyung Sik

AU - Chung, Hee Yong

AU - Choi, Inho

AU - Cheon, Yong Pil

AU - Lee, Ki Ho

AU - Lee, Dong Mok

AU - Park, Jongsun

AU - Lee, Sang Yeol

AU - Chun, Taehoon

N1 - Funding Information: This study was supported by Technology Development Program for Agriculture and Forestry (project number: 108089-3), Ministry for Agriculture, Forestry and Fisheries, Republic of Korea (to T. Chun) and a grant from Biogreen 21 project (project number: 20070401-034-026-007-06), Rural Development Administration, Republic of Korea (to H.-S. Kang).

PY - 2010/1/15

Y1 - 2010/1/15

N2 - Lymphocyte activation gene-3 (LAG-3; CD223) is a co-receptor that may function as a down-modulator of T cell activity via an interaction with MHC class II molecules. In this study, we cloned and sequenced the complete cDNA sequence of pig Lag3. Pig Lag3 cDNA contains an open reading frame (1524 bp) encoding 507 amino acids. The putative amino acid identity of pig LAG-3 with those of the human, rat, and mouse is 78%, 66%, and 67%, respectively. Pig Lag3 mRNA transcript was mainly expressed in lymphoid tissues, and was inducible upon stimulation of phytohemagglutinin (PHA). Next, we developed mouse antiserum against the extracellular domain (D1 and D2 domains) of pig LAG-3. Pig LAG-3 is detected as an approximately 67 kDa of a single species by Western blot analysis. Moreover, flow cytometry analysis confirmed that LAG-3 on pig T cells (CD3+ cells) is inducible upon PHA stimulation. These results indicated that the structure and expression pattern of pig LAG-3 are conserved among mammalian species.

AB - Lymphocyte activation gene-3 (LAG-3; CD223) is a co-receptor that may function as a down-modulator of T cell activity via an interaction with MHC class II molecules. In this study, we cloned and sequenced the complete cDNA sequence of pig Lag3. Pig Lag3 cDNA contains an open reading frame (1524 bp) encoding 507 amino acids. The putative amino acid identity of pig LAG-3 with those of the human, rat, and mouse is 78%, 66%, and 67%, respectively. Pig Lag3 mRNA transcript was mainly expressed in lymphoid tissues, and was inducible upon stimulation of phytohemagglutinin (PHA). Next, we developed mouse antiserum against the extracellular domain (D1 and D2 domains) of pig LAG-3. Pig LAG-3 is detected as an approximately 67 kDa of a single species by Western blot analysis. Moreover, flow cytometry analysis confirmed that LAG-3 on pig T cells (CD3+ cells) is inducible upon PHA stimulation. These results indicated that the structure and expression pattern of pig LAG-3 are conserved among mammalian species.

KW - Co-receptor

KW - Immune homeostasis

KW - Lymphocyte activation gene-3

KW - Pig

KW - T cell activation

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JO - Veterinary Immunology and Immunopathology

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Molecular cloning and expression analysis of pig lymphocyte activation gene-3 (LAG-3; CD223)

Abstract

Keywords

ASJC Scopus subject areas

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