Molecular cloning and expression of human keratinocyte proline-rich protein (hKPRP), an epidermal marker isolated from calcium-induced differentiating keratinocytes

Woong Hee Lee, Sunhyae Jang, Jung Suk Lee, Young Lee, Eun Young Seo, Kwan Hee You, Seung Chul Lee, Kwang Il Nam, Jin Man Kim, Sun Ho Kee, Jun Mo Yang, Young Joon Seo, Jang Kyu Park, Deok Kim Chang, Jeung Hoon Lee

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

We isolated a human gene encoding keratinocyte proline-rich protein (hKPRP). hKPRP gene is located in the region of epidermal differentiation complex on chromosome 1q21, and its ∼ 2.5 kb mRNA encodes 579 amino acid protein with high proline content (18%). The mRNA level of hKPRP was markedly increased at both 7 and 14 d after treatment with 1.2 mM calcium in cultured normal human epidermal keratinocytes. In situ hybridization demonstrated that hKPRP was expressed in upper granular layer of normal epidermis with characteristic intermittent pattern. In psoriatic lesion, hKPRP expression was increased as compared with normal skin and showed continuous pattern. Immunohistochemical analysis also confirmed the expression of hKPRP at the protein level. Western blot analysis showed that hKPRP protein of ∼ 70 kDa size was significantly increased by calcium in a time-dependent manner. In mouse tissue blot assays, the expression of KPRP was detected in stomach and skin tissues, and began at 17.5 embryonic days. Additionally, hKPRP expression was detected in the periderm of human fetal skin from 16 wk estimated gestational age. Together, these results suggest that hKPRP is an epidermal marker expressed in stratified squamous epithelia and has a potential role in keratinocytes differentiation.

Original languageEnglish
Pages (from-to)995-1000
Number of pages6
JournalJournal of Investigative Dermatology
Volume125
Issue number5
DOIs
Publication statusPublished - 2005 Nov

Keywords

  • Calcium
  • Epidermal differentiation
  • Human keratinocyte proline-rich protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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