Molecular cloning and expression of Turkey inhibin-α and -βa subunits

J. Ahn, Seungkwon You, Hyunggee Kim, D. N. Foster, M. E. El Halawani

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We isolated cDNA encoding turkey inhibin-α (tINH-α) and -βA (tINH-βA) subunits from the turkey ovary using reverse transcription-polymerase chain reaction (RT-PCR). The isolated α subunit and βA subunit included the entire open reading frames encoding 329 and 424 amino acids, respectively. The amino acid sequences of mature tINH-α subunit and tINH-βA subunit (12.6 and 12.9 kDa proteins, respectively), established via DNA sequence analysis, were highly conserved between the chicken and various mammals. Northern blot analysis revealed that the transcripts of tINH-α and tINH-βA subunits were approximately 1.7 and 8.4 kb, respectively. In various stages of follicular development, tINH-α mRNA was highly expressed in small white follicles as compared to postovulatory and regressed follicles, whereas tINH-βA mRNA was predominately expressed in preovulatory F5 follicles.

Original languageEnglish
Pages (from-to)1690-1694
Number of pages5
JournalPoultry Science
Volume80
Issue number12
Publication statusPublished - 2001 Dec 1
Externally publishedYes

Fingerprint

inhibin
Inhibins
Molecular Cloning
Turkey
molecular cloning
Turkey (country)
Messenger RNA
follicular development
DNA Sequence Analysis
Northern blotting
Northern Blotting
Open Reading Frames
Reverse Transcription
open reading frames
Mammals
Amino Acid Sequence
Ovary
Chickens
amino acid sequences
sequence analysis

Keywords

  • Subunit-βa
  • Turkey inhibin-α

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Ahn, J., You, S., Kim, H., Foster, D. N., & El Halawani, M. E. (2001). Molecular cloning and expression of Turkey inhibin-α and -βa subunits. Poultry Science, 80(12), 1690-1694.

Molecular cloning and expression of Turkey inhibin-α and -βa subunits. / Ahn, J.; You, Seungkwon; Kim, Hyunggee; Foster, D. N.; El Halawani, M. E.

In: Poultry Science, Vol. 80, No. 12, 01.12.2001, p. 1690-1694.

Research output: Contribution to journalArticle

Ahn, J, You, S, Kim, H, Foster, DN & El Halawani, ME 2001, 'Molecular cloning and expression of Turkey inhibin-α and -βa subunits', Poultry Science, vol. 80, no. 12, pp. 1690-1694.
Ahn, J. ; You, Seungkwon ; Kim, Hyunggee ; Foster, D. N. ; El Halawani, M. E. / Molecular cloning and expression of Turkey inhibin-α and -βa subunits. In: Poultry Science. 2001 ; Vol. 80, No. 12. pp. 1690-1694.
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