Molecular cloning, expression, and characterization of angiopoietin- related protein. Angiopoietin-related protein induces endothelial cell sprouting

Injune Kim, Sang Ok Moon, Keum Nim Koh, Hyun Kim, Chang Sub Uhm, Hee Jin Kwak, Nam Gyun Kim, Gou Young Koh

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Using degenerate polymerase chain reaction, we isolated a cDNA encoding a novel 493-amino acid protein from human and mouse adult heart cDNAs and have designated it angiopoietin-related protein-2 (ARP2). The NH2-terminal and COOH-terminal portions of ARP2 contain the characteristic coiled-coil domain and fibrinogen-like domain that are conserved in angiopoietins. ARP2 has two consensus glycosylation sites and a highly hydrophobic region at the NH2 terminus that is typical of a secretory signal sequence. Recombinant ARP2 expressed in COS cells is secreted and glycosylated. In human adult tissues, ARP2 mRNA is most abundant in heart, small intestine, spleen, and stomach. In rat embryos, ARP2 mRNA is most abundant in the blood vessels and skeletal muscles. Endothelial and vascular smooth muscle cells also contain ARP2 mRNA. Recombinant ARP2 protein induces sprouting in vascular endothelial cells but does not bind to the Tie1 or Tie2 receptor. These results suggest that ARP2 may exert a function on endothelial cells through autocrine or paracrine action.

Original languageEnglish
Pages (from-to)26523-26528
Number of pages6
JournalJournal of Biological Chemistry
Volume274
Issue number37
DOIs
Publication statusPublished - 1999 Sep 10

Fingerprint

Angiopoietins
Cloning
Endothelial cells
Molecular Cloning
Endothelial Cells
Proteins
Messenger RNA
Muscle
TIE-2 Receptor
Complementary DNA
Glycosylation
COS Cells
Polymerase chain reaction
Blood vessels
Protein Sorting Signals
Vascular Smooth Muscle
Fibrinogen
Small Intestine
Smooth Muscle Myocytes
Blood Vessels

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular cloning, expression, and characterization of angiopoietin- related protein. Angiopoietin-related protein induces endothelial cell sprouting. / Kim, Injune; Moon, Sang Ok; Koh, Keum Nim; Kim, Hyun; Uhm, Chang Sub; Kwak, Hee Jin; Kim, Nam Gyun; Koh, Gou Young.

In: Journal of Biological Chemistry, Vol. 274, No. 37, 10.09.1999, p. 26523-26528.

Research output: Contribution to journalArticle

Kim, Injune ; Moon, Sang Ok ; Koh, Keum Nim ; Kim, Hyun ; Uhm, Chang Sub ; Kwak, Hee Jin ; Kim, Nam Gyun ; Koh, Gou Young. / Molecular cloning, expression, and characterization of angiopoietin- related protein. Angiopoietin-related protein induces endothelial cell sprouting. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 37. pp. 26523-26528.
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