Molecular cloning of a novel pathogen-inducible cDNA encoding a putative acyl-CoA synthetase from Capsicum annuum L

Sang Jik Lee, Mi Chung Suh, Shinje Kim, Jin Kyung Kwon, Minwoo Kim, Kyung Hee Paek, Doil Choi, Byung Dong Kim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

By means of differential display, a pool of salicylic acid (SA)-induced mRNAs were identified and subsequently their cDNAs were isolated from a cDNA library prepared from SA-induced leaf tissues of hot pepper. One of these cDNA clones, designated CaSIG4, was 1900 bp and contained an open reading frame encoding 523 amino acids with a calculated molecular mass of 56.3 kDa. The predicted amino acid sequence of CaSIG4 showed high sequence similarity to the AMP-binding protein family of both prokaryotic and eukaryotic acyl-CoA synthetases. CaSIG4 transcripts accumulated rapidly after SA treatment and in response to both incompatible and compatible interactions with Xanthomonas campestris pv. vesicatoria race 1. To investigate the cis-acting elements mediating CaSIG4 expression, the CaSIG4 5′-flanking region was isolated by inverse PCR. Database searches indicated that a potential cis-regulatory element is almost identical to the consensus core sequences ACC(A/T)ACC(A/C) which are conserved among promoters of other phenylpropanoid biosynthetic genes. The subcellular localization of the CaSIG4 protein was studied by using a soluble modified GFP gene fusion delivered into epidermal cells of onion by biolistic bombardment. The CaSIG4-smGFP fusion protein was localized to the plasma membrane. Taken together, CaSIG4 encoding a putative acyl-CoA synthetase could function as a plasma membrane-bound protein with a role in signaling in plant defense.

Original languageEnglish
Pages (from-to)661-671
Number of pages11
JournalPlant Molecular Biology
Volume46
Issue number6
DOIs
Publication statusPublished - 2001

Keywords

  • AMP-binding protein
  • Acyl-CoA synthetase
  • Capsicum annuum L
  • Salicylic acid
  • Subcellular localization
  • mRNA differential display

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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