Molecular cloning of multiple splicing variants of JIP-1 preferentially expressed in brain

In Jung Kim, Ko Woon Lee, Byung Young Park, Ja Kyeong Lee, Jihyun Park, In Young Choi, Soo Jung Eom, Tong Shin Chang, Myung Jin Kim, Young Il Yeom, Sung Key Chang, Young Don Lee, Eui Ju Choi, Pyung Lim Han

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) is activated by a variety of cellular or environmental stresses. Proper regulation of the SAPK/JNK pathway may be critical for cell survival or death under various conditions. In this study, we report the molecular cloning of novel isoforms of JIP-1, which harbor a putative phosphotyrosine interaction domain and a helix-loop-helix domain, as well as an SH3 homologous region in the C terminus. Northern analysis indicates that transcription variant jip-1 is expressed in brain and kidney and transcription variants jip-2 and jip-3 are specifically expressed in brain. In situ hybridization data showed that the hybridized jip messages were heavily concentrated in adult brain, and were particularly enriched in the cerebral cortex and hippocampus, the brain regions vulnerable to pathological states such as hypoxia-ischemia, epilepsy, and Alzheimer's disease. All the deduced protein products of the jip transcription variants appear to have a similar property in that they inhibit the SAPK/JNK stimulation when overexpressed. Inhibition of SAPK activation by overexpression of the novel isoform JIP-2a resulted in suppression of etoposide-induced cell death in a neuroglioma cell line, N18TG. These findings suggest that JIP may play an important role in regulation of the SAPK pathway that is involved in stress-induced cellular responses.

Original languageEnglish
Pages (from-to)1335-1343
Number of pages9
JournalJournal of Neurochemistry
Volume72
Issue number4
DOIs
Publication statusPublished - 1999 Mar 31

Fingerprint

Cloning
Molecular Cloning
JNK Mitogen-Activated Protein Kinases
Brain
Transcription
Heat-Shock Proteins
Protein Kinases
Protein Isoforms
Helix-Loop-Helix Motifs
Cell Death
Cells
Phosphotyrosine
Etoposide
Cell death
Ports and harbors
Cerebral Cortex
In Situ Hybridization
Epilepsy
Hippocampus
Cell Survival

Keywords

  • Anti-cell death function
  • Apoptosis
  • Brain expression
  • JIP isoforms
  • SAPK/JNK

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Kim, I. J., Lee, K. W., Park, B. Y., Lee, J. K., Park, J., Choi, I. Y., ... Han, P. L. (1999). Molecular cloning of multiple splicing variants of JIP-1 preferentially expressed in brain. Journal of Neurochemistry, 72(4), 1335-1343. https://doi.org/10.1046/j.1471-4159.1999.721335.x

Molecular cloning of multiple splicing variants of JIP-1 preferentially expressed in brain. / Kim, In Jung; Lee, Ko Woon; Park, Byung Young; Lee, Ja Kyeong; Park, Jihyun; Choi, In Young; Eom, Soo Jung; Chang, Tong Shin; Kim, Myung Jin; Yeom, Young Il; Chang, Sung Key; Lee, Young Don; Choi, Eui Ju; Han, Pyung Lim.

In: Journal of Neurochemistry, Vol. 72, No. 4, 31.03.1999, p. 1335-1343.

Research output: Contribution to journalArticle

Kim, IJ, Lee, KW, Park, BY, Lee, JK, Park, J, Choi, IY, Eom, SJ, Chang, TS, Kim, MJ, Yeom, YI, Chang, SK, Lee, YD, Choi, EJ & Han, PL 1999, 'Molecular cloning of multiple splicing variants of JIP-1 preferentially expressed in brain', Journal of Neurochemistry, vol. 72, no. 4, pp. 1335-1343. https://doi.org/10.1046/j.1471-4159.1999.721335.x
Kim, In Jung ; Lee, Ko Woon ; Park, Byung Young ; Lee, Ja Kyeong ; Park, Jihyun ; Choi, In Young ; Eom, Soo Jung ; Chang, Tong Shin ; Kim, Myung Jin ; Yeom, Young Il ; Chang, Sung Key ; Lee, Young Don ; Choi, Eui Ju ; Han, Pyung Lim. / Molecular cloning of multiple splicing variants of JIP-1 preferentially expressed in brain. In: Journal of Neurochemistry. 1999 ; Vol. 72, No. 4. pp. 1335-1343.
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