Molecular cloning of neuronally expressed mouse βPix isoforms

Seyun Kim, Taeho Kim, Deokjae Lee, Sun Hwa Park, Hyun Kim, Dongeun Park

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Pix, a Pak-interacting exchange factor, is known to be involved in the regulation of Cdc42/Rac GTPases and Pak kinase activity. In this study, we cloned the cDNAs encoding two βPix isoforms from mouse brain cDNA library. Both of the cloned genes, designated βPix-b and βPix-c (GenBank Accession Nos. AF247654 and AF247655, respectively), have a novel insert region consisting of 59 amino acid residues. In βPix-c, 75 amino acid residues are deleted in the proline-rich region at the carboxyl-terminus of βPix. In situ hybridization studies with insert region-specific probe in rat embryo show that insert region-containing isoforms are expressed mainly in the central nervous system. Moreover, temporal expression pattern of isoforms is correlated with the active neurogenesis period in the cerebral cortex and cerebellum. These results strongly suggest that βPix isoforms may play important roles in the cellular events required for brain development such as neuronal migration. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)721-725
Number of pages5
JournalBiochemical and biophysical research communications
Volume272
Issue number3
DOIs
Publication statusPublished - 2000 Jun 16

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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