Molecular cytogenetic analysis of the monoblastic cell line U937: Karyotype clarification by G-banding, whole chromosome painting, microdissection and reverse painting, and comparative genomic hybridization

Ji-Yun Lee, Chul Hoon Lee, Sung Han Shim, Han Kyu Seo, Jee Hong Kyhm, Sechin Cho, Youl Hee Cho

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Previous reports on the analysis of the human monoblastic cell line U937 had described several sublines containing unidentified rearrangements and marker chromosomes. In order to determine the true nature of the rearrangements, conventional banding analysis was carried out with various combinations of molecular cytogenetic techniques: comparative genomic hybridization, fluorescence in situ hybridization (FISH) with whole chromosome painting probes, and microdissection and reverse painting FISH. The origins of the marker chromosomes were identified and the composite karyotype is described.

Original languageEnglish
Pages (from-to)124-132
Number of pages9
JournalCancer Genetics and Cytogenetics
Volume137
Issue number2
DOIs
Publication statusPublished - 2002 Sep 1
Externally publishedYes

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Chromosome Painting
Microdissection
Paintings
Comparative Genomic Hybridization
Cytogenetic Analysis
Fluorescence In Situ Hybridization
Karyotype
Genetic Markers
Cell Line
In Situ Hybridization

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Molecular cytogenetic analysis of the monoblastic cell line U937 : Karyotype clarification by G-banding, whole chromosome painting, microdissection and reverse painting, and comparative genomic hybridization. / Lee, Ji-Yun; Lee, Chul Hoon; Shim, Sung Han; Seo, Han Kyu; Kyhm, Jee Hong; Cho, Sechin; Cho, Youl Hee.

In: Cancer Genetics and Cytogenetics, Vol. 137, No. 2, 01.09.2002, p. 124-132.

Research output: Contribution to journalArticle

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