Molecular dissection of the interaction between hBLT2 and the G protein alpha subunits

Krishna Moorthy Vukoti, Won Kyu Lee, Ho Jun Kim, Ick Young Kim, Gyeong Yang Eun, Cheolju Lee, Gyu Yu Yeon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various G α protein including G αi1, G αi2, G αi3, G αs1, G αo1, and G αz. Among the tested G α subunits, G αi3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (KD) of 5.0 × 10-6 M. These results suggest that G αi3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with G αi3.

Original languageEnglish
Pages (from-to)1005-1009
Number of pages5
JournalBulletin of the Korean Chemical Society
Volume28
Issue number6
Publication statusPublished - 2007 Jun 20

Fingerprint

GTP-Binding Protein alpha Subunits
Dissection
Leukotriene B4
Chemotactic Factors
Pertussis Toxin
G-Protein-Coupled Receptors
GTP-Binding Proteins
Fusion reactions
Cells
Proteins
human LTB4R2 protein

Keywords

  • G-protein
  • GPCR-G protein interaction
  • hBLT2
  • Intracellular loop
  • Leukotriene B4

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Vukoti, K. M., Lee, W. K., Kim, H. J., Kim, I. Y., Eun, G. Y., Lee, C., & Yeon, G. Y. (2007). Molecular dissection of the interaction between hBLT2 and the G protein alpha subunits. Bulletin of the Korean Chemical Society, 28(6), 1005-1009.

Molecular dissection of the interaction between hBLT2 and the G protein alpha subunits. / Vukoti, Krishna Moorthy; Lee, Won Kyu; Kim, Ho Jun; Kim, Ick Young; Eun, Gyeong Yang; Lee, Cheolju; Yeon, Gyu Yu.

In: Bulletin of the Korean Chemical Society, Vol. 28, No. 6, 20.06.2007, p. 1005-1009.

Research output: Contribution to journalArticle

Vukoti, KM, Lee, WK, Kim, HJ, Kim, IY, Eun, GY, Lee, C & Yeon, GY 2007, 'Molecular dissection of the interaction between hBLT2 and the G protein alpha subunits', Bulletin of the Korean Chemical Society, vol. 28, no. 6, pp. 1005-1009.
Vukoti, Krishna Moorthy ; Lee, Won Kyu ; Kim, Ho Jun ; Kim, Ick Young ; Eun, Gyeong Yang ; Lee, Cheolju ; Yeon, Gyu Yu. / Molecular dissection of the interaction between hBLT2 and the G protein alpha subunits. In: Bulletin of the Korean Chemical Society. 2007 ; Vol. 28, No. 6. pp. 1005-1009.
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AB - Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various G α protein including G αi1, G αi2, G αi3, G αs1, G αo1, and G αz. Among the tested G α subunits, G αi3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (KD) of 5.0 × 10-6 M. These results suggest that G αi3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with G αi3.

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