Molecular genetic analysis of reovirus isolated in Korea

Jae Bok Lee, Young Eun Lee, Sang Hyun Kim, Jong Hun Kim, Kazuo Shirouzu, Kwang Sook Park, Luck Ju Baek, Jin Won Song, Ki Joon Song

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Reovirus isolates from human, striped-field mouse (Apodemus agrarius) and Korean field mouse (A. peninsulae) in Korea showed extensive variability in the patterns of electrophoretic migration of the double-stranded RNA (dsRNA) genome segments. Hemagglutination inhibition (HI) test was performed for serotype determination of 12 reovirus isolates. To clarify genetic diversity and molecular phylogeny of Korean reoviruses, L1, S3 and S4 genomic segments of reoviruses were amplified by RT-PCR and directly sequenced. Among 12 reovirus strains, 9 strains were type 3 and 3 strains were type 2. The L1 was highly conserved showing 91.5-100%, 94.7-100% similarities among Korean isolates, and 77.5-97.9%, 92.6-96.8% similarities compared to other reference strains of each genotypes at nucleotide and amino acid levels, respectively. In S3 and S4 segments, 84.4-99.3%, 72.3-99% nucleotide sequence similarities and 92-99.3%, 89.1-98.4% amino acid sequence similarities among Korean isolates were observed, and 70.8-93.9%, 72.3-98.7% nucleotide sequence similarities and 81.8-100%, 88.3-97.7% amino acid sequence similarities compared with other reference strains of each genotype were observed, respectively. Phylogenetic analyses based on the S3 and S4 nucleotide sequences indicate that genotypes of reovirus are more related with geographic differences rather than host species or date of isolation.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalKurume Medical Journal
Volume48
Issue number1
DOIs
Publication statusPublished - 2001

Keywords

  • Korea
  • Reovirus

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Lee, J. B., Lee, Y. E., Kim, S. H., Kim, J. H., Shirouzu, K., Park, K. S., Baek, L. J., Song, J. W., & Song, K. J. (2001). Molecular genetic analysis of reovirus isolated in Korea. Kurume Medical Journal, 48(1), 79-85. https://doi.org/10.2739/kurumemedj.48.79