Abstract
CD44 is a member of cell surface glycoproteins which are involved in cell-matrix adhesion and tumor metastasis. Certain types of tumors express complex CD44 isoforms generated by alternative splicing of 2v-10v exons, and their expression appears to promote metastasis of tumor cells. Using a nested RT-PCR, we analyzed expression of CD44 variants in 26 stomach carcinoma, 21 matched normal tissues, and 2 carcinoma cell lines. We observed frequent and complex patterns of CD44 variant expression in tumor tissues. While exons 6v and 7v expression was detected in most normal and tumor tissues, exon 9v was most rarely detected. Exon 5v showed a significantly frequent expression in carcinoma, suggesting that its expression might contribute to the malignant progression. While exon 9v was frequently observed in diffuse-type tumors, the other 8 variant exons including 6v showed more frequent expression in intestinal-type tumors. Exons 9v and 10v were predominantly expressed in advanced tumor tissues and exon 8v was expressed more frequently in tumors of lymph node metastasis. We believe that series with a longer follow-up now need to be tested to clarify the association between CD44 splice variant expression and distant metastasis or long-term prognosis.
| Original language | English |
|---|---|
| Pages (from-to) | 505-513 |
| Number of pages | 9 |
| Journal | Journal of Korean Medical Science |
| Volume | 12 |
| Issue number | 6 |
| Publication status | Published - 1997 Dec 1 |
| Externally published | Yes |
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Keywords
- Antigens, CD44
- Neoplasm metastasis
- Stomach neoplasms
ASJC Scopus subject areas
- Medicine(all)
Cite this
Molecular genetic characterization of alternatively spliced CD44 transcripts in human stomach carcinoma. / Kim, Yong Sik; Chi, Sung-Gil; Kim, Youn Wha; Park, Yong Koo; Yoon, Choong.
In: Journal of Korean Medical Science, Vol. 12, No. 6, 01.12.1997, p. 505-513.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular genetic characterization of alternatively spliced CD44 transcripts in human stomach carcinoma
AU - Kim, Yong Sik
AU - Chi, Sung-Gil
AU - Kim, Youn Wha
AU - Park, Yong Koo
AU - Yoon, Choong
PY - 1997/12/1
Y1 - 1997/12/1
N2 - CD44 is a member of cell surface glycoproteins which are involved in cell-matrix adhesion and tumor metastasis. Certain types of tumors express complex CD44 isoforms generated by alternative splicing of 2v-10v exons, and their expression appears to promote metastasis of tumor cells. Using a nested RT-PCR, we analyzed expression of CD44 variants in 26 stomach carcinoma, 21 matched normal tissues, and 2 carcinoma cell lines. We observed frequent and complex patterns of CD44 variant expression in tumor tissues. While exons 6v and 7v expression was detected in most normal and tumor tissues, exon 9v was most rarely detected. Exon 5v showed a significantly frequent expression in carcinoma, suggesting that its expression might contribute to the malignant progression. While exon 9v was frequently observed in diffuse-type tumors, the other 8 variant exons including 6v showed more frequent expression in intestinal-type tumors. Exons 9v and 10v were predominantly expressed in advanced tumor tissues and exon 8v was expressed more frequently in tumors of lymph node metastasis. We believe that series with a longer follow-up now need to be tested to clarify the association between CD44 splice variant expression and distant metastasis or long-term prognosis.
AB - CD44 is a member of cell surface glycoproteins which are involved in cell-matrix adhesion and tumor metastasis. Certain types of tumors express complex CD44 isoforms generated by alternative splicing of 2v-10v exons, and their expression appears to promote metastasis of tumor cells. Using a nested RT-PCR, we analyzed expression of CD44 variants in 26 stomach carcinoma, 21 matched normal tissues, and 2 carcinoma cell lines. We observed frequent and complex patterns of CD44 variant expression in tumor tissues. While exons 6v and 7v expression was detected in most normal and tumor tissues, exon 9v was most rarely detected. Exon 5v showed a significantly frequent expression in carcinoma, suggesting that its expression might contribute to the malignant progression. While exon 9v was frequently observed in diffuse-type tumors, the other 8 variant exons including 6v showed more frequent expression in intestinal-type tumors. Exons 9v and 10v were predominantly expressed in advanced tumor tissues and exon 8v was expressed more frequently in tumors of lymph node metastasis. We believe that series with a longer follow-up now need to be tested to clarify the association between CD44 splice variant expression and distant metastasis or long-term prognosis.
KW - Antigens, CD44
KW - Neoplasm metastasis
KW - Stomach neoplasms
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UR - http://www.scopus.com/inward/citedby.url?scp=0031298392&partnerID=8YFLogxK
M3 - Article
C2 - 9443088
AN - SCOPUS:0031298392
VL - 12
SP - 505
EP - 513
JO - Journal of Korean Medical Science
JF - Journal of Korean Medical Science
SN - 1011-8934
IS - 6
ER -