Molecular imaging of cell death in an experimental model of Parkinson's disease with a novel apoptosis-targeting peptide

Min Jeong Lee, Kai Wang, In-San Kim, Byung Heon Lee, Hyung Soo Han

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: We used a novel apoptosis-targeting peptide called ApoPep-1 in order to evaluate whether ApoPep-1 can be used as a diagnostic indicator in a model of Parkinson's disease (PD). Procedures: 1-Methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP) was given to mice to produce the PD model. Cy7.5-labeled ApoPep-1 was given intravenously and optical imaging was taken at 1, 2, and 3 weeks after MPTP treatment. Immunohistochemical study was performed with brain sections. Results: Increased ApoPep-1 signal was observed in the brain of MPTP-treated mice by in vivo and ex vivo imaging study. With histological evaluation, ApoPep-1 signal demonstrated a strong correlation with loss of dopaminergic neurons or increase of apoptotic cells. Moreover, the neuroprotective effect of amantadine in the MPTP model was effectively evaluated using optical imaging of ApoPep-1. Conclusions: We conclude that ApoPep-1 is the effective probe for imaging of apoptosis in the MPTP model and can be applied in brain diseases with apoptosis.

Original languageEnglish
Pages (from-to)147-155
Number of pages9
JournalMolecular Imaging and Biology
Volume14
Issue number2
DOIs
Publication statusPublished - 2012 Apr 1
Externally publishedYes

Fingerprint

Molecular Imaging
Parkinsonian Disorders
Cell Death
Theoretical Models
Apoptosis
Peptides
Optical Imaging
Parkinson Disease
Amantadine
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Dopaminergic Neurons
Brain
Brain Diseases
Neuroprotective Agents
cysteinyl-glutaminyl-arginyl-prolyl-prolyl-arginine
4-phenyl-1,2,3,6-tetrahydropyridine

Keywords

  • 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
  • Apoptosis
  • Apoptosis-targeting peptide
  • In vivo imaging
  • Parkinson's disease

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Molecular imaging of cell death in an experimental model of Parkinson's disease with a novel apoptosis-targeting peptide. / Lee, Min Jeong; Wang, Kai; Kim, In-San; Lee, Byung Heon; Han, Hyung Soo.

In: Molecular Imaging and Biology, Vol. 14, No. 2, 01.04.2012, p. 147-155.

Research output: Contribution to journalArticle

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