Molecular marker-assisted detection of rice weevil in stored rice

Hae Jun Jeong, Jee Eun Oh, Suk Whan Hong, Mun Il Ryoo, Ki Deok Kim, Hojoung Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Stored cereal crops form the major component of the world's food supply. It is well known that post-harvest crop losses, due to insects and microbes, cause significant economic waste in both developed and developing countries. In general, the environment in storage facilities is prone to rapid pest development, leading to significant grain losses. Rice weevil (Sitophilus oryzae L.) is one pest of cereal crops that deposits its eggs on or in a seed. To detect rice weevil in stored rice, we developed PCR-based molecular markers of Sitophilus oryzae. Several sets of PCR primers from the 'internal transcribed spacer 2′ gene of S. oryzae were analyzed to identify molecular markers that are specific to the S. oryzae genome. Among six sets of molecular markers identified, three were found to be useful for identifying rice weevil. Our results demonstrate that a PCR marker system permits rapid and reliable identification of weevil in stored grain. Furthermore, this PCR assay would expand the choice of tools for monitoring populations of the weevil in pest management programmes.

Original languageEnglish
Pages (from-to)325-331
Number of pages7
JournalInternational Journal of Pest Management
Volume52
Issue number4
DOIs
Publication statusPublished - 2006 Oct

Keywords

  • Oryza sativa
  • Rice weevil
  • Sitophilus oryzae
  • Sitophilus zeamais

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

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