Molecular mechanism involved in the response to hydrogen peroxide stress in Acinetobacter oleivorans DR1

Jisun Kim, Youjung Cho, In Ae Jang, Woojun Park

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Two-dimensional gel electrophoresis was conducted to investigate the effect of H2O2 on whole protein expression in Acinetobacter oleivorans DR1. Functional classification of 13 upregulated proteins using MALDI-TOF mass spectrometry showed relationships with oxidative stress, energy production and conversion, nucleotide and amino acid metabolism, membrane-related, ion transport, and chaperone-related functions. Alignment of OxyR-binding regions from Pseudomonas aeruginosa and Escherichia coli with promoters of identified proteins revealed that only ahpC, ahpF, and trxB (thioredoxin-disulfide reductase) genes, along with a newly found oprC (putative outer membrane receptor protein) gene, have OxyR-binding sites. The oxyR and ahpC mutants were more sensitive to H2O2 and showed growth defects in both nutritional and n-hexadecane-amended media. Four catalases present in the genome of A. oleivorans DR1 were not detected, which led us to confirm the expression and activity of those catalases in the presence of H2O2. The expression patterns of the four catalase genes differed at different concentrations of H2O2. Interestingly, the promoters of both known OxyR-controlled katG gene (AOLE_17390) and putative small catalase gene (AOLE_09800) have OxyR-binding sites. Gel-shift assay confirmed OxyR binding to the promoter regions of newly identified OxyR-controlled genes encoding OprC and a putative catalase. Hierarchical expression and OxyR-binding of several OxyR-controlled genes suggested that concentration is an important factor in inducing the set of genes under H2O2 stress.

Original languageEnglish
Pages (from-to)10611-10626
Number of pages16
JournalApplied Microbiology and Biotechnology
Volume99
Issue number24
DOIs
Publication statusPublished - 2015 Dec 1

Fingerprint

Acinetobacter
Hydrogen Peroxide
Catalase
Genes
Binding Sites
Thioredoxin-Disulfide Reductase
Proteins
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Ion Transport
Electrophoresis, Gel, Two-Dimensional
Genetic Promoter Regions
Pseudomonas aeruginosa
Mass Spectrometry
Membrane Proteins
Oxidative Stress
Nucleotides
Gels
Genome
Escherichia coli
Amino Acids

Keywords

  • Acinetobacter
  • HO
  • Oxidative stress
  • OxyR
  • Proteomics

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Molecular mechanism involved in the response to hydrogen peroxide stress in Acinetobacter oleivorans DR1. / Kim, Jisun; Cho, Youjung; Jang, In Ae; Park, Woojun.

In: Applied Microbiology and Biotechnology, Vol. 99, No. 24, 01.12.2015, p. 10611-10626.

Research output: Contribution to journalArticle

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