Molecular mechanisms underlying calcium current modulation by nociceptin

Kyu Young Yeon, Mi Young Sim, Se Young Choi, Sung Joong Lee, Kyungpyo Park, Joong Soo Kim, Jong Heun Lee, Kyung Mi Lee, Seog Bae Oh

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Nociceptin is a non-opioid peptide that modulates pain response. One of mechanism underlying its analgesic action is the inhibition of voltage-dependent calcium current (ICa), similar to that of opioids. We investigated the molecular mechanism by which nociceptin inhibits Ica using sensory neurons and a heterologous expression system. Ica inhibition by nociceptin was voltage-dependent, exhibited the slowing of activation kinetics and prepulse facilitation, and was blocked by N-ethylmaleimide, indicating the involvement of Gi/Go protein. ICa inhibition by nociceptin was primarily mediated through binding to its own receptor, ORL-1, but not through affecting other μ-opioid receptors. Thus, our results strongly demonstrate that heterologous cross-talk between ORL1 and μOR is not involved in the ICa inhibition by nociceptin.

Original languageEnglish
Pages (from-to)2205-2209
Number of pages5
JournalNeuroreport
Volume15
Issue number14
DOIs
Publication statusPublished - 2004 Oct 5

Keywords

  • Calcium currents
  • DAMGO
  • Nociceptin
  • ORL-1
  • Trigeminal ganglion neurons
  • μOR

ASJC Scopus subject areas

  • Neuroscience(all)

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