Molecular mechanisms underlying calcium current modulation by nociceptin

Kyu Young Yeon, Mi Young Sim, Se Young Choi, Sung Joong Lee, Kyungpyo Park, Joong Soo Kim, Jong Heun Lee, Kyung-Mi Lee, Seog Bae Oh

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Nociceptin is a non-opioid peptide that modulates pain response. One of mechanism underlying its analgesic action is the inhibition of voltage-dependent calcium current (ICa), similar to that of opioids. We investigated the molecular mechanism by which nociceptin inhibits Ica using sensory neurons and a heterologous expression system. Ica inhibition by nociceptin was voltage-dependent, exhibited the slowing of activation kinetics and prepulse facilitation, and was blocked by N-ethylmaleimide, indicating the involvement of Gi/Go protein. ICa inhibition by nociceptin was primarily mediated through binding to its own receptor, ORL-1, but not through affecting other μ-opioid receptors. Thus, our results strongly demonstrate that heterologous cross-talk between ORL1 and μOR is not involved in the ICa inhibition by nociceptin.

Original languageEnglish
Pages (from-to)2205-2209
Number of pages5
JournalNeuroReport
Volume15
Issue number14
DOIs
Publication statusPublished - 2004 Oct 5

Keywords

  • μOR
  • Calcium currents
  • DAMGO
  • Nociceptin
  • ORL-1
  • Trigeminal ganglion neurons

ASJC Scopus subject areas

  • Neuroscience(all)

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  • Cite this

    Yeon, K. Y., Sim, M. Y., Choi, S. Y., Lee, S. J., Park, K., Kim, J. S., Lee, J. H., Lee, K-M., & Oh, S. B. (2004). Molecular mechanisms underlying calcium current modulation by nociceptin. NeuroReport, 15(14), 2205-2209. https://doi.org/10.1097/00001756-200410050-00012