Momilactione B inhibits protein kinase A signaling and reduces tyrosinase-related proteins 1 and 2 expression in melanocytes

Ji Hae Lee, Boram Cho, Hee jin Jun, Woo Duck Seo, Dong Woo Kim, Kang Jin Cho, Sung-Joon Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Momilactone B (MB) is a terpenoid phytoalexin present in rice bran that exhibits several biological activities. MB reduced the melanin content in B16 melanocytes melanin content and inhibited tyrosinase activities. Using transcriptome analysis, the genes involved in protein kinase A (PKA) signaling were found to be markedly altered. B16 cells stimulated with MB had decreased concentrations of cAMP protein kinase A activity, and cAMP-response element-binding protein which is a key transcription factor for microphthalmia-associated transcription factor (MITF) expression. Accordingly, the expression of MITF and its target genes, which are essential for melanogenesis, were reduced. MB thus exhibits anti-melanogenic effects by repressing tyrosinase enzyme activity and inhibiting the PKA signaling pathway which, in turn, decreases melanogenic gene expression.

Original languageEnglish
Pages (from-to)805-812
Number of pages8
JournalBiotechnology Letters
Volume34
Issue number5
DOIs
Publication statusPublished - 2012 May 1

Fingerprint

Microphthalmia-Associated Transcription Factor
Melanocytes
Cyclic AMP-Dependent Protein Kinases
Monophenol Monooxygenase
Melanins
Cyclic AMP Response Element-Binding Protein
Essential Genes
Terpenes
Gene Expression Profiling
Gene Expression
tyrosinase-related protein-1
momilactone B
dopachrome isomerase
Enzymes
Genes

Keywords

  • Melanogenesis
  • Momilactone B
  • Protein kinase A
  • Tyrosinase

ASJC Scopus subject areas

  • Biotechnology

Cite this

Momilactione B inhibits protein kinase A signaling and reduces tyrosinase-related proteins 1 and 2 expression in melanocytes. / Lee, Ji Hae; Cho, Boram; Jun, Hee jin; Seo, Woo Duck; Kim, Dong Woo; Cho, Kang Jin; Lee, Sung-Joon.

In: Biotechnology Letters, Vol. 34, No. 5, 01.05.2012, p. 805-812.

Research output: Contribution to journalArticle

Lee, Ji Hae ; Cho, Boram ; Jun, Hee jin ; Seo, Woo Duck ; Kim, Dong Woo ; Cho, Kang Jin ; Lee, Sung-Joon. / Momilactione B inhibits protein kinase A signaling and reduces tyrosinase-related proteins 1 and 2 expression in melanocytes. In: Biotechnology Letters. 2012 ; Vol. 34, No. 5. pp. 805-812.
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AU - Kim, Dong Woo

AU - Cho, Kang Jin

AU - Lee, Sung-Joon

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