Monitoring antibiotic residues and corresponding antibiotic resistance genes in an agroecosystem

Yasser M. Awad, Kwon Rae Kim, Sung Chul Kim, Kangjoo Kim, Sang Ryong Lee, Sang Soo Lee, Yong Sik Ok

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Antibiotic resistance genes (ARGs) have been commonly reported due to the overuse worldwide of antibiotics. Antibiotic overuse disturbs the environment and threatens public human health. The objective of this study was to measure the residual concentrations of veterinary antibiotics in the tetracycline group (TCs), including tetracycline (TC) and chlortetracycline (CTC), as well as those in the sulfonamide group (SAs), including sulfamethazine (SMT), sulfamethoxazole (SMX), and sulfathiazole (STZ). We also isolated the corresponding ARGs in the agroecosystem. Four sediment samples and two rice paddy soil samples were collected from sites near a swine composting facility along the Naerincheon River in Hongcheon, Korea. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed with a solid-phase extraction method to measure the concentration of each antibiotic. ARGs were identified by the qualitative polymerase chain-reaction using synthetic primers. SAs and their corresponding ARGs were highly detected in sediment samples whereas TCs were not detected except for sediments sample #1. ARGs for TCs and SAs were detected in rice paddy soils, while ARGs for TCs were only found in sediment #2 and #4. Continuous monitoring of antibiotic residue and its comprehensive impact on the environment is needed to ensure environmental health.

Original languageEnglish
Article number974843
JournalJournal of Chemistry
Volume2015
DOIs
Publication statusPublished - 2015 Jan 1
Externally publishedYes

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Genes
Anti-Bacterial Agents
Monitoring
Sulfonamides
Sediments
Tetracycline
Sulfamethazine
Health
Soils
Sulfamethoxazole
Chlortetracycline
Composting
Polymerase chain reaction
High performance liquid chromatography
Mass spectrometry
Rivers

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Monitoring antibiotic residues and corresponding antibiotic resistance genes in an agroecosystem. / Awad, Yasser M.; Kim, Kwon Rae; Kim, Sung Chul; Kim, Kangjoo; Lee, Sang Ryong; Lee, Sang Soo; Ok, Yong Sik.

In: Journal of Chemistry, Vol. 2015, 974843, 01.01.2015.

Research output: Contribution to journalArticle

Awad, Yasser M. ; Kim, Kwon Rae ; Kim, Sung Chul ; Kim, Kangjoo ; Lee, Sang Ryong ; Lee, Sang Soo ; Ok, Yong Sik. / Monitoring antibiotic residues and corresponding antibiotic resistance genes in an agroecosystem. In: Journal of Chemistry. 2015 ; Vol. 2015.
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