Abstract
This study aims to develop a window chamber system in the skin of rats and to monitor the primo vascular system (PVS) inside the lymphatic vessels along the superficial epigastric vessels. The PVS in lymphatic vessels has been observed through many experiments under in vivo conditions, but monitoring the in vivo PVS in situ inside lymphatic vessels for a long time is difficult. To overcome the obstacles, we adapted the window chamber system for monitoring the PVS and Alcian blue (AB) staining dye solution for the contrast agent. The lymphatic vessels in the skin on the lateral side of the body, connecting the inguinal lymph nodes to the axillary lymph nodes, were the targets for setting the window system. After AB had been injected into the inguinal lymph nodes with a glass capillary, the morphological changes of the stained PVS were monitored through the window system for up to twenty hours, and the changes in the AB intensity in the PVS were quantified by using image processing. The results and histological images are presented in this study.
| Original language | English |
|---|---|
| Pages (from-to) | 1251-1259 |
| Number of pages | 9 |
| Journal | Biomedical Optics Express |
| Volume | 7 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2016 Mar 14 |
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ASJC Scopus subject areas
- Biotechnology
- Atomic and Molecular Physics, and Optics
Cite this
Monitoring the primo vascular system in lymphatic vessels by using window chambers. / Kim, Jungdae; Kim, Dong Hyun; Jung, Sharon Jiyoon; Gil, Hyun Ji; Yoon, Seung-Zhoo; Kim, Young Il; Soh, Kwang Sup.
In: Biomedical Optics Express, Vol. 7, No. 4, 14.03.2016, p. 1251-1259.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Monitoring the primo vascular system in lymphatic vessels by using window chambers
AU - Kim, Jungdae
AU - Kim, Dong Hyun
AU - Jung, Sharon Jiyoon
AU - Gil, Hyun Ji
AU - Yoon, Seung-Zhoo
AU - Kim, Young Il
AU - Soh, Kwang Sup
PY - 2016/3/14
Y1 - 2016/3/14
N2 - This study aims to develop a window chamber system in the skin of rats and to monitor the primo vascular system (PVS) inside the lymphatic vessels along the superficial epigastric vessels. The PVS in lymphatic vessels has been observed through many experiments under in vivo conditions, but monitoring the in vivo PVS in situ inside lymphatic vessels for a long time is difficult. To overcome the obstacles, we adapted the window chamber system for monitoring the PVS and Alcian blue (AB) staining dye solution for the contrast agent. The lymphatic vessels in the skin on the lateral side of the body, connecting the inguinal lymph nodes to the axillary lymph nodes, were the targets for setting the window system. After AB had been injected into the inguinal lymph nodes with a glass capillary, the morphological changes of the stained PVS were monitored through the window system for up to twenty hours, and the changes in the AB intensity in the PVS were quantified by using image processing. The results and histological images are presented in this study.
AB - This study aims to develop a window chamber system in the skin of rats and to monitor the primo vascular system (PVS) inside the lymphatic vessels along the superficial epigastric vessels. The PVS in lymphatic vessels has been observed through many experiments under in vivo conditions, but monitoring the in vivo PVS in situ inside lymphatic vessels for a long time is difficult. To overcome the obstacles, we adapted the window chamber system for monitoring the PVS and Alcian blue (AB) staining dye solution for the contrast agent. The lymphatic vessels in the skin on the lateral side of the body, connecting the inguinal lymph nodes to the axillary lymph nodes, were the targets for setting the window system. After AB had been injected into the inguinal lymph nodes with a glass capillary, the morphological changes of the stained PVS were monitored through the window system for up to twenty hours, and the changes in the AB intensity in the PVS were quantified by using image processing. The results and histological images are presented in this study.
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U2 - 10.1364/BOE.7.001251
DO - 10.1364/BOE.7.001251
M3 - Article
VL - 7
SP - 1251
EP - 1259
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 4
ER -