Monitoring the primo vascular system in lymphatic vessels by using window chambers

Jungdae Kim, Dong Hyun Kim, Sharon Jiyoon Jung, Hyun Ji Gil, Seung Zhoo Yoon, Young Il Kim, Kwang Sup Soh

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    This study aims to develop a window chamber system in the skin of rats and to monitor the primo vascular system (PVS) inside the lymphatic vessels along the superficial epigastric vessels. The PVS in lymphatic vessels has been observed through many experiments under in vivo conditions, but monitoring the in vivo PVS in situ inside lymphatic vessels for a long time is difficult. To overcome the obstacles, we adapted the window chamber system for monitoring the PVS and Alcian blue (AB) staining dye solution for the contrast agent. The lymphatic vessels in the skin on the lateral side of the body, connecting the inguinal lymph nodes to the axillary lymph nodes, were the targets for setting the window system. After AB had been injected into the inguinal lymph nodes with a glass capillary, the morphological changes of the stained PVS were monitored through the window system for up to twenty hours, and the changes in the AB intensity in the PVS were quantified by using image processing. The results and histological images are presented in this study.

    Original languageEnglish
    Pages (from-to)1251-1259
    Number of pages9
    JournalBiomedical Optics Express
    Volume7
    Issue number4
    DOIs
    Publication statusPublished - 2016 Mar 14

    ASJC Scopus subject areas

    • Biotechnology
    • Atomic and Molecular Physics, and Optics

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