Morphine postconditioning attenuates ICAM-1 expression on endothelial cells

Too Jae Min, Joong il Kim, Jae Hwan Kim, Kyung Hee Noh, Tae Woo Kim, Woon Young Kim, Yoon Sook Lee, Young Cheol Park

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The purpose of this study is to determine 1) whether morphine postconditiong (MPostC) can attenuate the intercellular adhesion molecules-1 (ICAM-1) expression after reoxygenation injury and 2) the subtype(s) of the opioid receptors (ORs) that are involved with MPostC. Human umbilical vein endothelial cells (HUVECs) were subjected to 6 hr anoxia followed by 12 hr reoxygenation. Three morphine concentrations (0.3, 3, 30 μM) were used to evaluate the protective effect of MPostC. We also investigated blockading the OR subtypes' effects on MPostC by using three antagonists (a μ-OR antagonist naloxone, a κ-OR antagonist nor-binaltorphimine, and a δ-OR antagonist naltrindole) and the inhibitor of protein kinase C (PKC) chelerythrine. As results, the ICAM-1 expression was significantly reduced in the MPostC (3, 30 μM) groups compared to the control group at 1, 6, 9, and 12 hours reoxygenation time. As a consequence, neutrophil adhesion was also decreased after MPostC. These effects were abolished by coadministering chelerythrine, norbinaltorphimine or naltrindole, but not with naloxone. In conclusion, it is assumed that MPostC could attenuate the expression of ICAM-1 on endothelial cells during reoxygenation via the κ and δ-OR (opioid receptor)-specific pathway, and this also involves a PKC-dependent pathway.

Original languageEnglish
Pages (from-to)290-296
Number of pages7
JournalJournal of Korean medical science
Volume26
Issue number2
DOIs
Publication statusPublished - 2011 Feb

Keywords

  • Cell culture
  • Endothelial cells
  • Humans
  • Morphine
  • Postconditioning
  • Reperfusion injury
  • Umblical veins

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Min, T. J., Kim, J. I., Kim, J. H., Noh, K. H., Kim, T. W., Kim, W. Y., Lee, Y. S., & Park, Y. C. (2011). Morphine postconditioning attenuates ICAM-1 expression on endothelial cells. Journal of Korean medical science, 26(2), 290-296. https://doi.org/10.3346/jkms.2011.26.2.290