Morphologic changes of zebrafish melanophore after intense pulsed light and Q-Switched Nd

YAG laser irradiation

Hwa Jung Ryu, Ji Min Lee, Hee Won Jang, Hae Chul Park, Im Joo Rhyu, Il-Hwan Kim

Research output: Contribution to journalArticle

Abstract

Background: Recently, the pulse-in-pulse mode of intense pulsed light (IPL) has been used increasingly for the treatment of melasma. Objective: To observe the morphologic changes in the melanophore in adult zebrafish after irradiation with conventional and pulse-in-pulse IPL and Q-switched Nd:YAG (QSNY) laser. Methods: Adult zebrafish were irradiated with conventional and pulse-in-pulse mode of IPL. The conditions for conventional IPL were 3 mJ/cm2, 560 nm filter, and pulse widths of 7, 20, and 35 msec. The pulse-in-pulse conditions were 3 mJ/cm2 and on-Time 1/off-Time 2. The QSNY laser was used with the settings of 1,064 nm, 0.4 J/cm2, a 7 mm spot size, and one shot. Specimens were observed using a light microscope, a transmission electron microscope (TEM), a scanning electron microscope (SEM) and a confocal microscope. Results: After conventional IPL irradiation with a 7 msec pulse width, melanophore breakage was observed using light microscopy. Under TEM, irradiation with conventional IPL for 7 msec and pulse-in-pulse IPL induced melanophore thermolysis with vacuolization. However, changes in the melanophore were not observed with 35 msec IPL. Under SEM, unlike the control and QSNY groups, IPL-irradiated zebrafish showed finger-like fusion in the protein structure of scales. Specimens examined by a confocal microscope after conventional IPL irradiation showed a larger green-stained area on TUNEL staining than that after pulse-in-pulse mode IPL irradiation. Conclusion: Zebrafish irradiated with long pulse-IPL showed no morphologic changes using light microscopy, while morphological changes in melanophores were evident with use of TEM. Pulse-in-pulse mode IPL caused less damage than conventional IPL.

Original languageEnglish
Pages (from-to)711-717
Number of pages7
JournalAnnals of Dermatology
Volume28
Issue number6
DOIs
Publication statusPublished - 2016 Dec 1

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Melanophores
Solid-State Lasers
Zebrafish
Light
Electrons
Microscopy
Melanosis

Keywords

  • Intense pulsed light
  • Melanophores
  • Zebrafish

ASJC Scopus subject areas

  • Dermatology

Cite this

Morphologic changes of zebrafish melanophore after intense pulsed light and Q-Switched Nd : YAG laser irradiation. / Ryu, Hwa Jung; Lee, Ji Min; Jang, Hee Won; Park, Hae Chul; Rhyu, Im Joo; Kim, Il-Hwan.

In: Annals of Dermatology, Vol. 28, No. 6, 01.12.2016, p. 711-717.

Research output: Contribution to journalArticle

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abstract = "Background: Recently, the pulse-in-pulse mode of intense pulsed light (IPL) has been used increasingly for the treatment of melasma. Objective: To observe the morphologic changes in the melanophore in adult zebrafish after irradiation with conventional and pulse-in-pulse IPL and Q-switched Nd:YAG (QSNY) laser. Methods: Adult zebrafish were irradiated with conventional and pulse-in-pulse mode of IPL. The conditions for conventional IPL were 3 mJ/cm2, 560 nm filter, and pulse widths of 7, 20, and 35 msec. The pulse-in-pulse conditions were 3 mJ/cm2 and on-Time 1/off-Time 2. The QSNY laser was used with the settings of 1,064 nm, 0.4 J/cm2, a 7 mm spot size, and one shot. Specimens were observed using a light microscope, a transmission electron microscope (TEM), a scanning electron microscope (SEM) and a confocal microscope. Results: After conventional IPL irradiation with a 7 msec pulse width, melanophore breakage was observed using light microscopy. Under TEM, irradiation with conventional IPL for 7 msec and pulse-in-pulse IPL induced melanophore thermolysis with vacuolization. However, changes in the melanophore were not observed with 35 msec IPL. Under SEM, unlike the control and QSNY groups, IPL-irradiated zebrafish showed finger-like fusion in the protein structure of scales. Specimens examined by a confocal microscope after conventional IPL irradiation showed a larger green-stained area on TUNEL staining than that after pulse-in-pulse mode IPL irradiation. Conclusion: Zebrafish irradiated with long pulse-IPL showed no morphologic changes using light microscopy, while morphological changes in melanophores were evident with use of TEM. Pulse-in-pulse mode IPL caused less damage than conventional IPL.",
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T2 - YAG laser irradiation

AU - Ryu, Hwa Jung

AU - Lee, Ji Min

AU - Jang, Hee Won

AU - Park, Hae Chul

AU - Rhyu, Im Joo

AU - Kim, Il-Hwan

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