Morphological and molecular characterization of the causal agent of downy mildew on quinoa (Chenopodium quinoa)

Young Joon Choi, Solveig Danielsen, Mette Lübeck, Seung Beom Hong, Rolf Delhey, Hyeon-Dong Shin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Downy mildew is an economically important and widespread disease in quinoa (Chenopodium quinoa) growing areas. Although in many studies Peronospora farinosa is most commonly regarded as the causal agent of the disease, identification and classification of the pathogen remain still uncertain due to its taxonomic confusion. Thirty-six Peronospora isolates from quinoa with different geographic origins including Argentina, Bolivia, Denmark, Ecuador, and Peru were morphologically and molecularly compared with Peronospora species from other Chenopodium species. The morphology of three herbarium specimens was similar to that of P. variabilis, which originated from C. album, characterized by flexuous to curved ultimate branchlets and pedicellated conidia. Phylogenetic analysis based on ITS rDNA sequences also placed the quinoa pathogen within the same clade as P. variabilis. Within the ITS rDNA sequences of the quinoa pathogens, two base substitutions were found, which separated the majority of the Danish isolates from isolates from South America, but no sequence difference was found among the isolates from different cultivars of quinoa. The present results indicate that the pathogen responsible for the quinoa downy mildew is identical to Peronospora variabilis and that it should not be lumped with P. farinosa as claimed previously by most studies.

Original languageEnglish
Pages (from-to)403-412
Number of pages10
JournalMycopathologia
Volume169
Issue number5
DOIs
Publication statusPublished - 2010 May 1

Fingerprint

Chenopodium quinoa
downy mildew
Peronospora
Peronospora farinosa
pathogens
Chenopodium
Ribosomal DNA
Chenopodium album
Bolivia
Ecuador
herbaria
Denmark
Peru
provenance
conidia
Argentina
Fungal Spores
taxonomy
South America
phylogeny

Keywords

  • Chenopodiaceae
  • Geographic origin
  • ITS rDNA
  • Morphology
  • Peronosporaceae
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Applied Microbiology and Biotechnology
  • Microbiology
  • veterinary (miscalleneous)

Cite this

Morphological and molecular characterization of the causal agent of downy mildew on quinoa (Chenopodium quinoa). / Choi, Young Joon; Danielsen, Solveig; Lübeck, Mette; Hong, Seung Beom; Delhey, Rolf; Shin, Hyeon-Dong.

In: Mycopathologia, Vol. 169, No. 5, 01.05.2010, p. 403-412.

Research output: Contribution to journalArticle

Choi, Young Joon ; Danielsen, Solveig ; Lübeck, Mette ; Hong, Seung Beom ; Delhey, Rolf ; Shin, Hyeon-Dong. / Morphological and molecular characterization of the causal agent of downy mildew on quinoa (Chenopodium quinoa). In: Mycopathologia. 2010 ; Vol. 169, No. 5. pp. 403-412.
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