Morphological characteristics of cordyceps sinensis 16 and production of mycelia and exo-biopolymer from molasses in submerged culture

Sun Hee Cha, Jong Chae Kim, Jung Soo Lim, Chul Sik Yoon, Jong Ho Koh, Hyo-Ihl Chang, Seung Wook Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The mycelial morphology and the production of mycelia and exo-biopolymer by Cordyceps sinensis 16 were investigated under different culture conditions. The mycelial morphology of C. sinensis 16 was characterized by image analysis. Some variations in morphological factors and exo-biopolymer production were observed under the different culture conditions, e.g., the initial pH and agitation speed. The initial pH had a significant effect on the morphology and on the production of mycelia and exo-biopolymer by C. sinensis 16 in submerged culture. At an initial pH of 5.0, the maximal hyphal length, the number of tips, and the mycelia concentration were determined. The agitation speed was an important factor for exo-biopolymer production in terms of controlling the growth and morphology of C. sinensis 16 in the bioreactor culture. In bioreactor cultures, as the agitation speed was increased, rapid differentiation and cell lysis occurred; at 350 rpm, a mycelial concentration of 62.3 g/L and an exo-biopolymer concentration of 22.0 g/L were obtained. This study demonstrates that, in submerged C. sinensis 16 cultures, morphological factors can be applied to efficiently produce mycelia and exo-biopolymer.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
JournalJournal of Industrial and Engineering Chemistry
Volume12
Issue number1
Publication statusPublished - 2006 Jan 1

Fingerprint

Molasses
Biopolymers
Bioreactors
Image analysis

Keywords

  • Cordyceps sinensis 16
  • Exo-biopolymer
  • Molasses
  • Morphology
  • Submerged culture

ASJC Scopus subject areas

  • Engineering (miscellaneous)

Cite this

Morphological characteristics of cordyceps sinensis 16 and production of mycelia and exo-biopolymer from molasses in submerged culture. / Cha, Sun Hee; Kim, Jong Chae; Lim, Jung Soo; Yoon, Chul Sik; Koh, Jong Ho; Chang, Hyo-Ihl; Kim, Seung Wook.

In: Journal of Industrial and Engineering Chemistry, Vol. 12, No. 1, 01.01.2006, p. 115-120.

Research output: Contribution to journalArticle

@article{25f1272b100245d19b276bf6c91ce959,
title = "Morphological characteristics of cordyceps sinensis 16 and production of mycelia and exo-biopolymer from molasses in submerged culture",
abstract = "The mycelial morphology and the production of mycelia and exo-biopolymer by Cordyceps sinensis 16 were investigated under different culture conditions. The mycelial morphology of C. sinensis 16 was characterized by image analysis. Some variations in morphological factors and exo-biopolymer production were observed under the different culture conditions, e.g., the initial pH and agitation speed. The initial pH had a significant effect on the morphology and on the production of mycelia and exo-biopolymer by C. sinensis 16 in submerged culture. At an initial pH of 5.0, the maximal hyphal length, the number of tips, and the mycelia concentration were determined. The agitation speed was an important factor for exo-biopolymer production in terms of controlling the growth and morphology of C. sinensis 16 in the bioreactor culture. In bioreactor cultures, as the agitation speed was increased, rapid differentiation and cell lysis occurred; at 350 rpm, a mycelial concentration of 62.3 g/L and an exo-biopolymer concentration of 22.0 g/L were obtained. This study demonstrates that, in submerged C. sinensis 16 cultures, morphological factors can be applied to efficiently produce mycelia and exo-biopolymer.",
keywords = "Cordyceps sinensis 16, Exo-biopolymer, Molasses, Morphology, Submerged culture",
author = "Cha, {Sun Hee} and Kim, {Jong Chae} and Lim, {Jung Soo} and Yoon, {Chul Sik} and Koh, {Jong Ho} and Hyo-Ihl Chang and Kim, {Seung Wook}",
year = "2006",
month = "1",
day = "1",
language = "English",
volume = "12",
pages = "115--120",
journal = "Journal of Industrial and Engineering Chemistry",
issn = "1226-086X",
publisher = "Korean Society of Industrial Engineering Chemistry",
number = "1",

}

TY - JOUR

T1 - Morphological characteristics of cordyceps sinensis 16 and production of mycelia and exo-biopolymer from molasses in submerged culture

AU - Cha, Sun Hee

AU - Kim, Jong Chae

AU - Lim, Jung Soo

AU - Yoon, Chul Sik

AU - Koh, Jong Ho

AU - Chang, Hyo-Ihl

AU - Kim, Seung Wook

PY - 2006/1/1

Y1 - 2006/1/1

N2 - The mycelial morphology and the production of mycelia and exo-biopolymer by Cordyceps sinensis 16 were investigated under different culture conditions. The mycelial morphology of C. sinensis 16 was characterized by image analysis. Some variations in morphological factors and exo-biopolymer production were observed under the different culture conditions, e.g., the initial pH and agitation speed. The initial pH had a significant effect on the morphology and on the production of mycelia and exo-biopolymer by C. sinensis 16 in submerged culture. At an initial pH of 5.0, the maximal hyphal length, the number of tips, and the mycelia concentration were determined. The agitation speed was an important factor for exo-biopolymer production in terms of controlling the growth and morphology of C. sinensis 16 in the bioreactor culture. In bioreactor cultures, as the agitation speed was increased, rapid differentiation and cell lysis occurred; at 350 rpm, a mycelial concentration of 62.3 g/L and an exo-biopolymer concentration of 22.0 g/L were obtained. This study demonstrates that, in submerged C. sinensis 16 cultures, morphological factors can be applied to efficiently produce mycelia and exo-biopolymer.

AB - The mycelial morphology and the production of mycelia and exo-biopolymer by Cordyceps sinensis 16 were investigated under different culture conditions. The mycelial morphology of C. sinensis 16 was characterized by image analysis. Some variations in morphological factors and exo-biopolymer production were observed under the different culture conditions, e.g., the initial pH and agitation speed. The initial pH had a significant effect on the morphology and on the production of mycelia and exo-biopolymer by C. sinensis 16 in submerged culture. At an initial pH of 5.0, the maximal hyphal length, the number of tips, and the mycelia concentration were determined. The agitation speed was an important factor for exo-biopolymer production in terms of controlling the growth and morphology of C. sinensis 16 in the bioreactor culture. In bioreactor cultures, as the agitation speed was increased, rapid differentiation and cell lysis occurred; at 350 rpm, a mycelial concentration of 62.3 g/L and an exo-biopolymer concentration of 22.0 g/L were obtained. This study demonstrates that, in submerged C. sinensis 16 cultures, morphological factors can be applied to efficiently produce mycelia and exo-biopolymer.

KW - Cordyceps sinensis 16

KW - Exo-biopolymer

KW - Molasses

KW - Morphology

KW - Submerged culture

UR - http://www.scopus.com/inward/record.url?scp=31944449991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=31944449991&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:31944449991

VL - 12

SP - 115

EP - 120

JO - Journal of Industrial and Engineering Chemistry

JF - Journal of Industrial and Engineering Chemistry

SN - 1226-086X

IS - 1

ER -