Morphology and kinetics studies on cephalosporin C production by Cephalosporium acremonium M25 in a 30-I bioreactor using a mixture of inocula

J. H. Kim, J. S. Lim, C. H. Kim, Seung Wook Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aims: In this study, the relationship between morphology and cephalosporin C (CPC) production in a 30-I bioreactor culture of Cephalosporium acremonium M25 using a 3:7 seed mixture was investigated. In addition, the kinetic model was established and applied. Methods and Results: CPC production was performed in a 30-I bioreactor using a 3:7 seed mixture. It was recognized that a 3:7 seed mixture was able to reduce lag phase and enhance CPC production. The maximum CPC production and cell mass were 1.96 and 81.5 g 1-1 respectively. Through a morphology study by observation using image analysis, it was concluded that changes of morphological features predicted the progressive production of CPC and that a morphology study could be useful in monitoring the CPC fermentation by C. acremonium M25. In the kinetics study, a kinetic model of CPC fermentation was developed and applied. The proposed model could adequately describe the fermentation of C. acremonium M25 in a 30-1 bioreactor. Conclusions: CPC productivity was improved by using a 3:7 seed mixture in a 30-1 bioreactor. The changes in morphological features showed a very similar tendency with CPC production. A kinetic model of CPC fermentation was successfully established. Significance and Impact of the Study: The results of the present study suggest that the use of a 3:7 seed mixture inocula has considerable possibilities for improving CPC productivity if applied to industrial scale fermentations. Through morphology and kinetics study, the kinetic model to describe the morphological differentiation and CPC production by C. acremonium M25 was established.

Original languageEnglish
Pages (from-to)307-311
Number of pages5
JournalLetters in Applied Microbiology
Volume40
Issue number5
DOIs
Publication statusPublished - 2005 May 6

Fingerprint

Acremonium
Bioreactors
bioreactor
Enzyme kinetics
fermentation
Fermentation
kinetics
Seed
Kinetics
seed
Seeds
Productivity
productivity
image analysis
cephalosporin C
Image analysis
monitoring
Monitoring

Keywords

  • Cephalosporium acremonium
  • Image analysis
  • Kinetics
  • Morphology

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Water Science and Technology
  • Fluid Flow and Transfer Processes
  • Microbiology
  • Ecology

Cite this

Morphology and kinetics studies on cephalosporin C production by Cephalosporium acremonium M25 in a 30-I bioreactor using a mixture of inocula. / Kim, J. H.; Lim, J. S.; Kim, C. H.; Kim, Seung Wook.

In: Letters in Applied Microbiology, Vol. 40, No. 5, 06.05.2005, p. 307-311.

Research output: Contribution to journalArticle

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