MoS2 field-effect transistor-amyloid‑β1−42 hybrid device for signal amplified detection of MMP‑9

Heekyeong Park, Hyungbeen Lee, Seok Hwan Jeong, Eunjin Lee, Wonseok Lee, Na Liu, Dae Sung Yoon, Sunkook Kim, Sang Woo Lee

Research output: Contribution to journalArticle

Abstract

The detection of circulating protein (CP) is very important for the diagnosis and therapeutics of cancer. Conventional techniques based on a specific antibody−antigen interaction are still lacking because of a shortage of cost effectiveness, complicated sandwich structure and tagging process, and inconsistent detection of CP due to the inherent instability of antibodies. Herein, we demonstrate a hybrid device consisting of two-dimensional (2D) nanoscale molybdenum disulfide (MoS2) field-effect transistor (FET) with an amyloid-β142 (Aβ142) functionalized surface, which amplifies electric signals of the FET in order to detect matrix metalloproteinase-9 (MMP-9), which is a certain type of CP that degrades Aβ1−42. With the hybrid device, we detected the concentrations of MMP-9 in the range from 1 pM to 10 nM. Moreover, using tapping-mode atomic force microscopy and Kelvin probe force microscopy, we verified that the signal amplification corresponding to the MMP-9 concentrations was caused by the reduced length and the decreased surface potential of degraded Aβ142 due to MMP-9. The hybrid device studied in this paper can be very useful for monitoring MMP-9 activity, as well as serving as a sensing platform for the electrical signal amplification of 2D MoS2 FET-biosensors.

Original languageEnglish
Pages (from-to)8252-8258
Number of pages7
JournalAnalytical chemistry
Volume91
Issue number13
DOIs
Publication statusPublished - 2019 Jan 1

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Matrix Metalloproteinase 9
Field effect transistors
Amyloid
Amplification
Amyloidogenic Proteins
Sandwich structures
Cost effectiveness
Biosensors
Atomic force microscopy
Microscopic examination
Proteins
Antibodies
Monitoring

ASJC Scopus subject areas

  • Analytical Chemistry

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MoS2 field-effect transistor-amyloid‑β1−42 hybrid device for signal amplified detection of MMP‑9. / Park, Heekyeong; Lee, Hyungbeen; Jeong, Seok Hwan; Lee, Eunjin; Lee, Wonseok; Liu, Na; Yoon, Dae Sung; Kim, Sunkook; Lee, Sang Woo.

In: Analytical chemistry, Vol. 91, No. 13, 01.01.2019, p. 8252-8258.

Research output: Contribution to journalArticle

Park, Heekyeong ; Lee, Hyungbeen ; Jeong, Seok Hwan ; Lee, Eunjin ; Lee, Wonseok ; Liu, Na ; Yoon, Dae Sung ; Kim, Sunkook ; Lee, Sang Woo. / MoS2 field-effect transistor-amyloid‑β1−42 hybrid device for signal amplified detection of MMP‑9. In: Analytical chemistry. 2019 ; Vol. 91, No. 13. pp. 8252-8258.
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