Mouse disabled 2 interacting protein 2 functions as a transcriptional repressor through direct binding onto its own promoter

Hyung Wook Kim, Young Joon Choi, Jung Ah Kim, Seong Ho Bae, Kyoung Rae Kim, Sung Soo Park

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The mDaIP2 protein is a mouse orthologue of human Nostrin (a regulator of eNos). The absence of eNos activity in RA-treated F9 cell implies that the protein plays somehow different role from Nostrin. In this experiment, this protein has been shown to repress the expression of its own gene, via a feedback mechanism which involves binding to the promoter region. Data from cotransfection, DNAP, mDaIP2-silenced F9 cell, and EMSA analyses clearly support the repression activity and direct binding of the protein to the promoter region. The protein contains N-terminal FCH domain and C-terminal SH3 domain. The SH3 domain is known to interact with the proline-rich domain of mDab2, while even no function has been reported about the FCH domain. Here, we report a novel function of mDaIP2 as a transcriptional repressor and suggest the possible association of the FCH domain with transcriptional regulation.

Original languageEnglish
Pages (from-to)75-81
Number of pages7
JournalBiochemical and biophysical research communications
Volume337
Issue number1
DOIs
Publication statusPublished - 2005 Nov 11

Keywords

  • Autogenous regulation
  • F9 teratocarcinoma cells
  • Transcriptional repression
  • mDaIP2

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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