Mouse Sprr2 genes: A clustered family of genes showing differential expression in epithelial tissues

Hae Jun Song, George Poy, Nadine Darwiche, Ulrike Lichti, Toshio Kuroki, Peter M. Steinert, Tonja Kartasova

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Small proline-rich (SPR) proteins are structural components of the cornified cell envelope of stratified squamous epithelia. They are subdivided into three families, i.e., SPR1, SPR2, and SPR3, of which the SPR2 family is the most complex. To understand the significance of this complexity, we have isolated 11 mouse Sprr2 genes, constructed a provisional physical map of the Sprr2 locus on mouse Chromosome 3, and examined the expression patterns of the Sprr2 genes in mouse epithelial tissues. The 11 Sprr2 sequences are highly conserved with a central domain containing a variable number of repeats. In situ hybridization showed the Sprr2 expression to be confined to epithelia. RT-PCR using primers specific for each of the 11 Sprr2 members demonstrated varying degrees of expression among the individual Sprr2 members in different tissues. The correlation between the physical location of the genes in the Sprr2 locus and their expression patterns suggests multiple levels of controlled expression.

Original languageEnglish
Pages (from-to)28-42
Number of pages15
JournalGenomics
Volume55
Issue number1
DOIs
Publication statusPublished - 1999 Jan 1
Externally publishedYes

Fingerprint

Epithelium
Genes
Chromosomes, Human, Pair 3
Conserved Sequence
Cellular Structures
Proline
In Situ Hybridization
Polymerase Chain Reaction
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Song, H. J., Poy, G., Darwiche, N., Lichti, U., Kuroki, T., Steinert, P. M., & Kartasova, T. (1999). Mouse Sprr2 genes: A clustered family of genes showing differential expression in epithelial tissues. Genomics, 55(1), 28-42. https://doi.org/10.1006/geno.1998.5607

Mouse Sprr2 genes : A clustered family of genes showing differential expression in epithelial tissues. / Song, Hae Jun; Poy, George; Darwiche, Nadine; Lichti, Ulrike; Kuroki, Toshio; Steinert, Peter M.; Kartasova, Tonja.

In: Genomics, Vol. 55, No. 1, 01.01.1999, p. 28-42.

Research output: Contribution to journalArticle

Song, HJ, Poy, G, Darwiche, N, Lichti, U, Kuroki, T, Steinert, PM & Kartasova, T 1999, 'Mouse Sprr2 genes: A clustered family of genes showing differential expression in epithelial tissues', Genomics, vol. 55, no. 1, pp. 28-42. https://doi.org/10.1006/geno.1998.5607
Song, Hae Jun ; Poy, George ; Darwiche, Nadine ; Lichti, Ulrike ; Kuroki, Toshio ; Steinert, Peter M. ; Kartasova, Tonja. / Mouse Sprr2 genes : A clustered family of genes showing differential expression in epithelial tissues. In: Genomics. 1999 ; Vol. 55, No. 1. pp. 28-42.
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