TY - JOUR
T1 - Mouse Sprr2 genes
T2 - A clustered family of genes showing differential expression in epithelial tissues
AU - Song, Hae Jun
AU - Poy, George
AU - Darwiche, Nadine
AU - Lichti, Ulrike
AU - Kuroki, Toshio
AU - Steinert, Peter M.
AU - Kartasova, Tonja
N1 - Funding Information:
We thank Drs Kiyoshi Nose, Motoko Shibanuma, Manabu Koike, Motoi Ohba, Nam-ho Huh, Yohko Kohno, and Shin-ichi Osada for valuable discussions and suggestions in the initial stages of this work. We are grateful to Drs Sherri Bale, Claude Backendorf, John Compton, Adam Glick, Elizabeth Joseloff, Gabriella Richards, and Stuart H. Yuspa for critically reading the manuscript. We thank Dr. Geraldine Rogers for help with the pulse-gel electrophoresis. Mr. E. Soban’s skills in frozen sectioning are highly appreciated. Mr. R. V. Dreyfuss of the Photography Section of the NIH is gratefully acknowledged for his excellent photographic services. Chris Remie provided invaluable help with graphic design and layout of figures. Tonja Kartasova was the recipient of a fellowship from Japan Society for Promotion of Science and of a senior research fellowship from the U.S. National Research Council. This work was partially supported by Shiseido Cosmetic Co.
PY - 1999/1/1
Y1 - 1999/1/1
N2 - Small proline-rich (SPR) proteins are structural components of the cornified cell envelope of stratified squamous epithelia. They are subdivided into three families, i.e., SPR1, SPR2, and SPR3, of which the SPR2 family is the most complex. To understand the significance of this complexity, we have isolated 11 mouse Sprr2 genes, constructed a provisional physical map of the Sprr2 locus on mouse Chromosome 3, and examined the expression patterns of the Sprr2 genes in mouse epithelial tissues. The 11 Sprr2 sequences are highly conserved with a central domain containing a variable number of repeats. In situ hybridization showed the Sprr2 expression to be confined to epithelia. RT-PCR using primers specific for each of the 11 Sprr2 members demonstrated varying degrees of expression among the individual Sprr2 members in different tissues. The correlation between the physical location of the genes in the Sprr2 locus and their expression patterns suggests multiple levels of controlled expression.
AB - Small proline-rich (SPR) proteins are structural components of the cornified cell envelope of stratified squamous epithelia. They are subdivided into three families, i.e., SPR1, SPR2, and SPR3, of which the SPR2 family is the most complex. To understand the significance of this complexity, we have isolated 11 mouse Sprr2 genes, constructed a provisional physical map of the Sprr2 locus on mouse Chromosome 3, and examined the expression patterns of the Sprr2 genes in mouse epithelial tissues. The 11 Sprr2 sequences are highly conserved with a central domain containing a variable number of repeats. In situ hybridization showed the Sprr2 expression to be confined to epithelia. RT-PCR using primers specific for each of the 11 Sprr2 members demonstrated varying degrees of expression among the individual Sprr2 members in different tissues. The correlation between the physical location of the genes in the Sprr2 locus and their expression patterns suggests multiple levels of controlled expression.
UR - http://www.scopus.com/inward/record.url?scp=0032912586&partnerID=8YFLogxK
U2 - 10.1006/geno.1998.5607
DO - 10.1006/geno.1998.5607
M3 - Article
C2 - 9888996
AN - SCOPUS:0032912586
SN - 0888-7543
VL - 55
SP - 28
EP - 42
JO - Genomics
JF - Genomics
IS - 1
ER -