MsrB1 and MICALs Regulate Actin Assembly and Macrophage Function via Reversible Stereoselective Methionine Oxidation

Byung Cheon Lee, Zalán Péterfi, Fu Kun W. Hoffmann, Richard E. Moore, Alaattin Kaya, Andrei Avanesov, Lionel Tarrago, Yani Zhou, Eranthie Weerapana, Dmitri E. Fomenko, Peter R. Hoffmann, Vadim N. Gladyshev

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Redox control of protein function involves oxidation and reduction of amino acid residues, but the mechanisms and regulators involved are insufficiently understood. Here, we report that in conjunction with Mical proteins, methionine-. R-sulfoxide reductase B1 (MsrB1) regulates mammalian actin assembly via stereoselective methionine oxidation and reduction in a reversible, site-specific manner. Two methionine residues in actin are specifically converted to methionine-. R-sulfoxide by Mical1 and Mical2 and reduced back to methionine by selenoprotein MsrB1, supporting actin disassembly and assembly, respectively. Macrophages utilize this redox control during cellular activation by stimulating MsrB1 expression and activity as a part of innate immunity. We identified the regulatory role of MsrB1 as a Mical antagonist in orchestrating actin dynamics and macrophage function. More generally, our study shows that proteins can be regulated by reversible site-specific methionine-. R-sulfoxidation.

Original languageEnglish
Pages (from-to)397-404
Number of pages8
JournalMolecular Cell
Volume51
Issue number3
DOIs
Publication statusPublished - 2013 Aug 8
Externally publishedYes

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Methionine
Actins
Macrophages
Oxidation-Reduction
sulfoxide
Selenoproteins
Proteins
Innate Immunity
Amino Acids

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Lee, B. C., Péterfi, Z., Hoffmann, F. K. W., Moore, R. E., Kaya, A., Avanesov, A., ... Gladyshev, V. N. (2013). MsrB1 and MICALs Regulate Actin Assembly and Macrophage Function via Reversible Stereoselective Methionine Oxidation. Molecular Cell, 51(3), 397-404. https://doi.org/10.1016/j.molcel.2013.06.019

MsrB1 and MICALs Regulate Actin Assembly and Macrophage Function via Reversible Stereoselective Methionine Oxidation. / Lee, Byung Cheon; Péterfi, Zalán; Hoffmann, Fu Kun W.; Moore, Richard E.; Kaya, Alaattin; Avanesov, Andrei; Tarrago, Lionel; Zhou, Yani; Weerapana, Eranthie; Fomenko, Dmitri E.; Hoffmann, Peter R.; Gladyshev, Vadim N.

In: Molecular Cell, Vol. 51, No. 3, 08.08.2013, p. 397-404.

Research output: Contribution to journalArticle

Lee, BC, Péterfi, Z, Hoffmann, FKW, Moore, RE, Kaya, A, Avanesov, A, Tarrago, L, Zhou, Y, Weerapana, E, Fomenko, DE, Hoffmann, PR & Gladyshev, VN 2013, 'MsrB1 and MICALs Regulate Actin Assembly and Macrophage Function via Reversible Stereoselective Methionine Oxidation', Molecular Cell, vol. 51, no. 3, pp. 397-404. https://doi.org/10.1016/j.molcel.2013.06.019
Lee, Byung Cheon ; Péterfi, Zalán ; Hoffmann, Fu Kun W. ; Moore, Richard E. ; Kaya, Alaattin ; Avanesov, Andrei ; Tarrago, Lionel ; Zhou, Yani ; Weerapana, Eranthie ; Fomenko, Dmitri E. ; Hoffmann, Peter R. ; Gladyshev, Vadim N. / MsrB1 and MICALs Regulate Actin Assembly and Macrophage Function via Reversible Stereoselective Methionine Oxidation. In: Molecular Cell. 2013 ; Vol. 51, No. 3. pp. 397-404.
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