Mucosal expression of ENaC and AQP in experimental otitis media induced by Eustachian tube obstruction

Jae-Jun Song, Seong Keun Kown, Eo Jin Kim, Yung Seop Lee, Bo Yeon Kim, Sungwon Chae

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective: We investigated the expression of the epithelial sodium channel (ENaC) and aquaporins (AQPs) in the middle ear mucosa of a rat model of otitis media with effusion caused by surgical obstruction of the Eustachian tube. Methods: Sixty-four rats were randomly assigned to either undergo unilateral Eustachian tube obstruction (groups 1, 2, and 3) or to undergo no procedure (control group). Bony Eustachian tubes were approached through ventral incisions and obstructed with electrocautery. On days 14, 28, and 56, the ears were evaluated, and the rats were sacrificed for otoscopic evaluation and real-time RT-PCR. Immunohistochemistry was done for ENaC-α and AQP-1. Results: The level of ENaC-α expression decreased 0.28- and 0.73-fold at 2 and 4 weeks, respectively, but increased 1.48-fold at 8 weeks (p < 0.05). The change in ENaC-β expression at 2 weeks was insignificant. However, the level of ENaC-β expression increased 3.17- and 7.85-fold at 4 and 8 weeks, respectively (p < 0.05). The level of ENaC-γ expression increased 1.51-, 4.82- and 14.79-fold at 2, 4 and 8 weeks, respectively (p < 0.05). The level of AQP-1 expression decreased 0.10- and 0.04-fold at 4 and 8 weeks, respectively (p < 0.05). The change in AQP4 expression at 4 and 8 weeks was insignificant (p > 0.05). The pattern of immunoreactivity of ENaC-α and AQP-1 was similar with that of gene expression. Conclusion: The experimental methods provoked reproducible otitis media with effusion. This model is well suited for studies of middle ear homeostasis during disease pathogenesis. Middle ear mucosa homeostasis is altered significantly by ETO, and the subunits of AQP proteins show a characteristic expression pattern over time.

Original languageEnglish
Pages (from-to)1589-1593
Number of pages5
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume73
Issue number11
DOIs
Publication statusPublished - 2009 Nov 1

Fingerprint

Eustachian Tube
Aquaporins
Otitis Media
Middle Ear
Aquaporin 1
Otitis Media with Effusion
Mucous Membrane
Homeostasis
Epithelial Sodium Channels
Electrocoagulation
Protein Subunits
Ear
Real-Time Polymerase Chain Reaction
Immunohistochemistry
Gene Expression
Control Groups

Keywords

  • Aquaporin
  • Epithelial sodium channel
  • Eustachian tube
  • Otitis media with effusion

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Otorhinolaryngology

Cite this

Mucosal expression of ENaC and AQP in experimental otitis media induced by Eustachian tube obstruction. / Song, Jae-Jun; Kown, Seong Keun; Kim, Eo Jin; Lee, Yung Seop; Kim, Bo Yeon; Chae, Sungwon.

In: International Journal of Pediatric Otorhinolaryngology, Vol. 73, No. 11, 01.11.2009, p. 1589-1593.

Research output: Contribution to journalArticle

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abstract = "Objective: We investigated the expression of the epithelial sodium channel (ENaC) and aquaporins (AQPs) in the middle ear mucosa of a rat model of otitis media with effusion caused by surgical obstruction of the Eustachian tube. Methods: Sixty-four rats were randomly assigned to either undergo unilateral Eustachian tube obstruction (groups 1, 2, and 3) or to undergo no procedure (control group). Bony Eustachian tubes were approached through ventral incisions and obstructed with electrocautery. On days 14, 28, and 56, the ears were evaluated, and the rats were sacrificed for otoscopic evaluation and real-time RT-PCR. Immunohistochemistry was done for ENaC-α and AQP-1. Results: The level of ENaC-α expression decreased 0.28- and 0.73-fold at 2 and 4 weeks, respectively, but increased 1.48-fold at 8 weeks (p < 0.05). The change in ENaC-β expression at 2 weeks was insignificant. However, the level of ENaC-β expression increased 3.17- and 7.85-fold at 4 and 8 weeks, respectively (p < 0.05). The level of ENaC-γ expression increased 1.51-, 4.82- and 14.79-fold at 2, 4 and 8 weeks, respectively (p < 0.05). The level of AQP-1 expression decreased 0.10- and 0.04-fold at 4 and 8 weeks, respectively (p < 0.05). The change in AQP4 expression at 4 and 8 weeks was insignificant (p > 0.05). The pattern of immunoreactivity of ENaC-α and AQP-1 was similar with that of gene expression. Conclusion: The experimental methods provoked reproducible otitis media with effusion. This model is well suited for studies of middle ear homeostasis during disease pathogenesis. Middle ear mucosa homeostasis is altered significantly by ETO, and the subunits of AQP proteins show a characteristic expression pattern over time.",
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AU - Kown, Seong Keun

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AU - Lee, Yung Seop

AU - Kim, Bo Yeon

AU - Chae, Sungwon

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AB - Objective: We investigated the expression of the epithelial sodium channel (ENaC) and aquaporins (AQPs) in the middle ear mucosa of a rat model of otitis media with effusion caused by surgical obstruction of the Eustachian tube. Methods: Sixty-four rats were randomly assigned to either undergo unilateral Eustachian tube obstruction (groups 1, 2, and 3) or to undergo no procedure (control group). Bony Eustachian tubes were approached through ventral incisions and obstructed with electrocautery. On days 14, 28, and 56, the ears were evaluated, and the rats were sacrificed for otoscopic evaluation and real-time RT-PCR. Immunohistochemistry was done for ENaC-α and AQP-1. Results: The level of ENaC-α expression decreased 0.28- and 0.73-fold at 2 and 4 weeks, respectively, but increased 1.48-fold at 8 weeks (p < 0.05). The change in ENaC-β expression at 2 weeks was insignificant. However, the level of ENaC-β expression increased 3.17- and 7.85-fold at 4 and 8 weeks, respectively (p < 0.05). The level of ENaC-γ expression increased 1.51-, 4.82- and 14.79-fold at 2, 4 and 8 weeks, respectively (p < 0.05). The level of AQP-1 expression decreased 0.10- and 0.04-fold at 4 and 8 weeks, respectively (p < 0.05). The change in AQP4 expression at 4 and 8 weeks was insignificant (p > 0.05). The pattern of immunoreactivity of ENaC-α and AQP-1 was similar with that of gene expression. Conclusion: The experimental methods provoked reproducible otitis media with effusion. This model is well suited for studies of middle ear homeostasis during disease pathogenesis. Middle ear mucosa homeostasis is altered significantly by ETO, and the subunits of AQP proteins show a characteristic expression pattern over time.

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