Muju virus, a novel hantavirus harboured by the arvicolid rodent Myodes regulus in Korea

Ki-Joon Song, Luck J. Baek, Sungsil Moon, Si Jung Ha, Sang Hyun Kim, Kwang Sook Park, Terry A. Klein, William Sames, Heung Chul Kim, John S. Lee, Richard Yanagihara, Jin Won Song

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Acute-phase sera from >5% of cases of haemorrhagic fever with renal syndrome occurring annually in Korea have been found to exhibit a fourfold or higher antibody titre to Puumala virus (PUUV) than to Hantaan virus (HTNV) by double-sandwich IgM ELISA, suggesting the existence of a PUUV-related hantavirus. Based on the phylogenetic relationships among arvicolid rodents, the royal vole (Myodes regulus) was targeted as a likely reservoir host of hantavirus. Using RT-PCR, a genetically distinct hantavirus, designated Muju virus (MUJV), was detected in lung tissue of royal voles, captured in widely separated geographical regions in Korea during 1996-2007. Pairwise analysis of the full-length S (1857 nt) and M (3634 nt) segments of MUJV indicated approximately 77% sequence similarity with PUUV. At the amino acid level, MUJV differed from PUUV by 5.5-6.9% (nucleocapsid) and 10.0-11.6% (Gn and Gc envelope glycoproteins). Interstrain variation of MUJV sequences from royal voles captured in different regions suggested geographic-specific clustering. Neutralizing antibody titres against PUUV were two- to sixfold higher than to HTNV in sera of MUJV-infected Myodes regulus. Although virus isolation attempts were unsuccessful, the collective data indicate that MUJV is a distinct hantavirus species.

Original languageEnglish
Pages (from-to)3121-3129
Number of pages9
JournalJournal of General Virology
Volume88
Issue number11
DOIs
Publication statusPublished - 2007 Nov 1

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Hantavirus
Arvicolinae
Puumala virus
Korea
Rodentia
Viruses
Hantaan virus
Hemorrhagic Fever with Renal Syndrome
Nucleocapsid
Neutralizing Antibodies
Serum
Immunoglobulin M
Cluster Analysis
Glycoproteins
Enzyme-Linked Immunosorbent Assay
Amino Acids
Polymerase Chain Reaction
Lung
Antibodies

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Muju virus, a novel hantavirus harboured by the arvicolid rodent Myodes regulus in Korea. / Song, Ki-Joon; Baek, Luck J.; Moon, Sungsil; Ha, Si Jung; Kim, Sang Hyun; Park, Kwang Sook; Klein, Terry A.; Sames, William; Kim, Heung Chul; Lee, John S.; Yanagihara, Richard; Song, Jin Won.

In: Journal of General Virology, Vol. 88, No. 11, 01.11.2007, p. 3121-3129.

Research output: Contribution to journalArticle

Song, K-J, Baek, LJ, Moon, S, Ha, SJ, Kim, SH, Park, KS, Klein, TA, Sames, W, Kim, HC, Lee, JS, Yanagihara, R & Song, JW 2007, 'Muju virus, a novel hantavirus harboured by the arvicolid rodent Myodes regulus in Korea', Journal of General Virology, vol. 88, no. 11, pp. 3121-3129. https://doi.org/10.1099/vir.0.83139-0
Song, Ki-Joon ; Baek, Luck J. ; Moon, Sungsil ; Ha, Si Jung ; Kim, Sang Hyun ; Park, Kwang Sook ; Klein, Terry A. ; Sames, William ; Kim, Heung Chul ; Lee, John S. ; Yanagihara, Richard ; Song, Jin Won. / Muju virus, a novel hantavirus harboured by the arvicolid rodent Myodes regulus in Korea. In: Journal of General Virology. 2007 ; Vol. 88, No. 11. pp. 3121-3129.
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