Multi-layered enzyme coating on highly conductive magnetic biochar nanoparticles for bisphenol A sensing in water

Lingzhi He, Yuesuo Yang, Jungbae Kim, Lan Yao, Xinwei Dong, Taihua Li, Yunxian Piao

Research output: Contribution to journalArticle

Abstract

This work introduced stable immobilization of tyrosinase (TYR) enzymes onto the highly conductive magnetic biochar nanoparticles with carboxyl functionality to construct sensitive and reusable electrochemical biosensor for bisphenol A (BPA) detection. The newly synthesized biochar nanoparticles (BCNPs) from biomass had outstanding electrochemical activity, after functionalization with carboxylic functional groups and magnetic nanoparticles (Mag-BCNPs-COOH), it was used for multi-layered TYR immobilization with covalent attachment, precipitation and cross-linking method (ML-TYR/Mag-BCNPs-COOH). The BPA sensing system was simply constructed by directly attaching the biocatalyst of ML-TYR/Mag-BCNPs-COOH onto a glassy carbon electrode with magnet force. Owning to great properties of the ML-TYR/Mag-BCNPs-COOH, such as high stability, biocatalytic activity and conductivity, the BPA sensing signal was significantly enhanced. And the lowest detection limit of 2.78 nM with linear ranges from 0.01 to 1.01 μM was achieved. The modified electrode could be reused at least eight cycles without reducing the sensing signal, and could be used for the real environmental water detection.

Original languageEnglish
Article number123276
JournalChemical Engineering Journal
Volume384
DOIs
Publication statusPublished - 2020 Mar 15

Fingerprint

coating
Monophenol Monooxygenase
Enzymes
enzyme
Nanoparticles
Coatings
Water
water
immobilization
electrode
Electrodes
Biocatalysts
Glassy carbon
biochar
bisphenol A
nanoparticle
Biosensors
Functional groups
functional group
Magnets

Keywords

  • Biosensor
  • Bisphenol A
  • Conductive biochar nanoparticles
  • Tyrosinase

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

Multi-layered enzyme coating on highly conductive magnetic biochar nanoparticles for bisphenol A sensing in water. / He, Lingzhi; Yang, Yuesuo; Kim, Jungbae; Yao, Lan; Dong, Xinwei; Li, Taihua; Piao, Yunxian.

In: Chemical Engineering Journal, Vol. 384, 123276, 15.03.2020.

Research output: Contribution to journalArticle

He, Lingzhi ; Yang, Yuesuo ; Kim, Jungbae ; Yao, Lan ; Dong, Xinwei ; Li, Taihua ; Piao, Yunxian. / Multi-layered enzyme coating on highly conductive magnetic biochar nanoparticles for bisphenol A sensing in water. In: Chemical Engineering Journal. 2020 ; Vol. 384.
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AU - Dong, Xinwei

AU - Li, Taihua

AU - Piao, Yunxian

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