TY - JOUR
T1 - Multi-level Integrative Biomarker Responses in Freshwater Teleostean Fishes Exposed to Almix Herbicide
AU - Samanta, Palas
AU - Pal, Sandipan
AU - Mukherjee, Aloke Kumar
AU - Senapati, Tarakeshwar
AU - Jung, Jinho
AU - Ghosh, Apurba Ratan
N1 - Funding Information:
Acknowledgements The authors like to thank the Department of Science and Technology, Govt. of India for the financial assistance through DST INSPIRE Fellowship Program (DST/INSPIRE Fellowship/2011/164, Dt. 29.09.2011) to Dr Palas Samanta. We also like to thank the Head, Department of Environmental Science, The University of Burdwan, Burdwan, West Bengal, India for providing the laboratory and library facilities during the course of research. Presently, Dr Samanta joined Korea University as Research Professor through BK21 Plus fellowship programme funded by the Ministry of Education of Korea.
Publisher Copyright:
© 2017, University of Tehran.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - In the present study, multi-level integrative biomarker responses (multi-level IBR) were studied to evaluate the toxicological effects of Almix herbicide towards Indian teleosts, namely Anabas testudineus, Heteropneustes fossilis and Oreochromis niloticus under laboratory (66.7 mg/L) and field (8 g/acre) conditions (30 days). At physiological level, condition factor (CF) displayed significant increase (p < 0.05) under both conditions, while liver somatic index (LSI) exhibited significant increase under field. Haematological parameters were significantly induced (enhanced and/or reduced), when compared with control value under both conditions in concerned fish species, although responses were species-specific. At molecular level, micronucleus (MN) study showed enhanced erythrocyte nuclear abnormalities (ENA) under both conditions; response was more severe under laboratory study [H. fossilis (0.37 ± 0.08) > O. niloticus (0.31 ± 0.06) > A. testudineus (0.23 ± 0.03)]. However, in field study, the degree of ENA were H. fossilis (0.14 ± 0.03) > O. niloticus (0.11 ± 0.04) > A. testudineus (0.09 ± 0.03). Additionally, multi-level IBR index indicating the degree of severity as of H. fossilis (6.97-fold) > A. testudineus (threefold) > O. niloticus (twofold) under laboratory condition, while in field study, it was H. fossilis (4.98-fold) > A. testudineus (twofold) > O. niloticus (1.67-fold). However, higher IBR index value in laboratory study indicated more severe adverse effects of Almix intoxication than field study, although varied multi-level IBR index under different conditions suggested the different contamination level. Therefore, these findings infer that multi-level IBR index might serve as a useful tool for quantitative monitoring of toxicological effects.
AB - In the present study, multi-level integrative biomarker responses (multi-level IBR) were studied to evaluate the toxicological effects of Almix herbicide towards Indian teleosts, namely Anabas testudineus, Heteropneustes fossilis and Oreochromis niloticus under laboratory (66.7 mg/L) and field (8 g/acre) conditions (30 days). At physiological level, condition factor (CF) displayed significant increase (p < 0.05) under both conditions, while liver somatic index (LSI) exhibited significant increase under field. Haematological parameters were significantly induced (enhanced and/or reduced), when compared with control value under both conditions in concerned fish species, although responses were species-specific. At molecular level, micronucleus (MN) study showed enhanced erythrocyte nuclear abnormalities (ENA) under both conditions; response was more severe under laboratory study [H. fossilis (0.37 ± 0.08) > O. niloticus (0.31 ± 0.06) > A. testudineus (0.23 ± 0.03)]. However, in field study, the degree of ENA were H. fossilis (0.14 ± 0.03) > O. niloticus (0.11 ± 0.04) > A. testudineus (0.09 ± 0.03). Additionally, multi-level IBR index indicating the degree of severity as of H. fossilis (6.97-fold) > A. testudineus (threefold) > O. niloticus (twofold) under laboratory condition, while in field study, it was H. fossilis (4.98-fold) > A. testudineus (twofold) > O. niloticus (1.67-fold). However, higher IBR index value in laboratory study indicated more severe adverse effects of Almix intoxication than field study, although varied multi-level IBR index under different conditions suggested the different contamination level. Therefore, these findings infer that multi-level IBR index might serve as a useful tool for quantitative monitoring of toxicological effects.
KW - Almix
KW - Erythrocyte nuclear abnormalities
KW - Field and laboratory conditions
KW - Haematology
KW - Micronucleus test
UR - http://www.scopus.com/inward/record.url?scp=85032018075&partnerID=8YFLogxK
U2 - 10.1007/s41742-017-0042-2
DO - 10.1007/s41742-017-0042-2
M3 - Article
AN - SCOPUS:85032018075
VL - 11
SP - 475
EP - 487
JO - International Journal of Environmental Research
JF - International Journal of Environmental Research
SN - 1735-6865
IS - 4
ER -