Abstract
In the present study, multi-level integrative biomarker responses (multi-level IBR) were studied to evaluate the toxicological effects of Almix herbicide towards Indian teleosts, namely Anabas testudineus, Heteropneustes fossilis and Oreochromis niloticus under laboratory (66.7 mg/L) and field (8 g/acre) conditions (30 days). At physiological level, condition factor (CF) displayed significant increase (p < 0.05) under both conditions, while liver somatic index (LSI) exhibited significant increase under field. Haematological parameters were significantly induced (enhanced and/or reduced), when compared with control value under both conditions in concerned fish species, although responses were species-specific. At molecular level, micronucleus (MN) study showed enhanced erythrocyte nuclear abnormalities (ENA) under both conditions; response was more severe under laboratory study [H. fossilis (0.37 ± 0.08) > O. niloticus (0.31 ± 0.06) > A. testudineus (0.23 ± 0.03)]. However, in field study, the degree of ENA were H. fossilis (0.14 ± 0.03) > O. niloticus (0.11 ± 0.04) > A. testudineus (0.09 ± 0.03). Additionally, multi-level IBR index indicating the degree of severity as of H. fossilis (6.97-fold) > A. testudineus (threefold) > O. niloticus (twofold) under laboratory condition, while in field study, it was H. fossilis (4.98-fold) > A. testudineus (twofold) > O. niloticus (1.67-fold). However, higher IBR index value in laboratory study indicated more severe adverse effects of Almix intoxication than field study, although varied multi-level IBR index under different conditions suggested the different contamination level. Therefore, these findings infer that multi-level IBR index might serve as a useful tool for quantitative monitoring of toxicological effects.
| Original language | English |
|---|---|
| Pages (from-to) | 475-487 |
| Number of pages | 13 |
| Journal | International Journal of Environmental Research |
| Volume | 11 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2017 Oct 1 |
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Keywords
- Almix
- Erythrocyte nuclear abnormalities
- Field and laboratory conditions
- Haematology
- Micronucleus test
ASJC Scopus subject areas
- Environmental Science(all)
Cite this
Multi-level Integrative Biomarker Responses in Freshwater Teleostean Fishes Exposed to Almix Herbicide. / Samanta, Palas; Pal, Sandipan; Mukherjee, Aloke Kumar; Senapati, Tarakeshwar; Jung, Jinho; Ghosh, Apurba Ratan.
In: International Journal of Environmental Research, Vol. 11, No. 4, 01.10.2017, p. 475-487.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multi-level Integrative Biomarker Responses in Freshwater Teleostean Fishes Exposed to Almix Herbicide
AU - Samanta, Palas
AU - Pal, Sandipan
AU - Mukherjee, Aloke Kumar
AU - Senapati, Tarakeshwar
AU - Jung, Jinho
AU - Ghosh, Apurba Ratan
PY - 2017/10/1
Y1 - 2017/10/1
N2 - In the present study, multi-level integrative biomarker responses (multi-level IBR) were studied to evaluate the toxicological effects of Almix herbicide towards Indian teleosts, namely Anabas testudineus, Heteropneustes fossilis and Oreochromis niloticus under laboratory (66.7 mg/L) and field (8 g/acre) conditions (30 days). At physiological level, condition factor (CF) displayed significant increase (p < 0.05) under both conditions, while liver somatic index (LSI) exhibited significant increase under field. Haematological parameters were significantly induced (enhanced and/or reduced), when compared with control value under both conditions in concerned fish species, although responses were species-specific. At molecular level, micronucleus (MN) study showed enhanced erythrocyte nuclear abnormalities (ENA) under both conditions; response was more severe under laboratory study [H. fossilis (0.37 ± 0.08) > O. niloticus (0.31 ± 0.06) > A. testudineus (0.23 ± 0.03)]. However, in field study, the degree of ENA were H. fossilis (0.14 ± 0.03) > O. niloticus (0.11 ± 0.04) > A. testudineus (0.09 ± 0.03). Additionally, multi-level IBR index indicating the degree of severity as of H. fossilis (6.97-fold) > A. testudineus (threefold) > O. niloticus (twofold) under laboratory condition, while in field study, it was H. fossilis (4.98-fold) > A. testudineus (twofold) > O. niloticus (1.67-fold). However, higher IBR index value in laboratory study indicated more severe adverse effects of Almix intoxication than field study, although varied multi-level IBR index under different conditions suggested the different contamination level. Therefore, these findings infer that multi-level IBR index might serve as a useful tool for quantitative monitoring of toxicological effects.
AB - In the present study, multi-level integrative biomarker responses (multi-level IBR) were studied to evaluate the toxicological effects of Almix herbicide towards Indian teleosts, namely Anabas testudineus, Heteropneustes fossilis and Oreochromis niloticus under laboratory (66.7 mg/L) and field (8 g/acre) conditions (30 days). At physiological level, condition factor (CF) displayed significant increase (p < 0.05) under both conditions, while liver somatic index (LSI) exhibited significant increase under field. Haematological parameters were significantly induced (enhanced and/or reduced), when compared with control value under both conditions in concerned fish species, although responses were species-specific. At molecular level, micronucleus (MN) study showed enhanced erythrocyte nuclear abnormalities (ENA) under both conditions; response was more severe under laboratory study [H. fossilis (0.37 ± 0.08) > O. niloticus (0.31 ± 0.06) > A. testudineus (0.23 ± 0.03)]. However, in field study, the degree of ENA were H. fossilis (0.14 ± 0.03) > O. niloticus (0.11 ± 0.04) > A. testudineus (0.09 ± 0.03). Additionally, multi-level IBR index indicating the degree of severity as of H. fossilis (6.97-fold) > A. testudineus (threefold) > O. niloticus (twofold) under laboratory condition, while in field study, it was H. fossilis (4.98-fold) > A. testudineus (twofold) > O. niloticus (1.67-fold). However, higher IBR index value in laboratory study indicated more severe adverse effects of Almix intoxication than field study, although varied multi-level IBR index under different conditions suggested the different contamination level. Therefore, these findings infer that multi-level IBR index might serve as a useful tool for quantitative monitoring of toxicological effects.
KW - Almix
KW - Erythrocyte nuclear abnormalities
KW - Field and laboratory conditions
KW - Haematology
KW - Micronucleus test
UR - http://www.scopus.com/inward/record.url?scp=85032018075&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85032018075&partnerID=8YFLogxK
U2 - 10.1007/s41742-017-0042-2
DO - 10.1007/s41742-017-0042-2
M3 - Article
AN - SCOPUS:85032018075
VL - 11
SP - 475
EP - 487
JO - International Journal of Environmental Research
JF - International Journal of Environmental Research
SN - 1735-6865
IS - 4
ER -