Multi-locus tree and species tree approaches toward resolving a complex clade of downy mildews (Straminipila, Oomycota), including pathogens of beet and spinach

Young Joon Choi, Steven J. Klosterman, Volker Kummer, Hermann Voglmayr, Hyeon-Dong Shin, Marco Thines

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Accurate species determination of plant pathogens is a prerequisite for their control and quarantine, and further for assessing their potential threat to crops. The family Peronosporaceae (Straminipila; Oomycota) consists of obligate biotrophic pathogens that cause downy mildew disease on angiosperms, including a large number of cultivated plants. In the largest downy mildew genus Peronospora, a phylogenetically complex clade includes the economically important downy mildew pathogens of spinach and beet, as well as the type species of the genus Peronospora. To resolve this complex clade at the species level and to infer evolutionary relationships among them, we used multi-locus phylogenetic analysis and species tree estimation. Both approaches discriminated all nine currently accepted species and revealed four previously unrecognized lineages, which are specific to a host genus or species. This is in line with a narrow species concept, i.e. that a downy mildew species is associated with only a particular host plant genus or species. Instead of applying the dubious name Peronospora farinosa, which has been proposed for formal rejection, our results provide strong evidence that Peronospora schachtii is an independent species from lineages on Atriplex and apparently occurs exclusively on Beta vulgaris. The members of the clade investigated, the Peronospora rumicis clade, associate with three different host plant families, Amaranthaceae, Caryophyllaceae, and Polygonaceae, suggesting that they may have speciated following at least two recent inter-family host shifts, rather than contemporary cospeciation with the host plants.

Original languageEnglish
Pages (from-to)24-34
Number of pages11
JournalMolecular Phylogenetics and Evolution
Volume86
DOIs
Publication statusPublished - 2015 May 1

Fingerprint

Peronospora
Oomycetes
mildews
Beta vulgaris
Spinacia oleracea
downy mildew
beets
spinach
pathogen
loci
host plants
pathogens
Peronospora farinosa
Atriplex
Polygonaceae
Caryophyllaceae
Amaranthaceae
host plant
Quarantine
Angiosperms

Keywords

  • Cospeciation
  • Host shift
  • Multi-locus phylogeny
  • Oomycetes
  • Peronospora farinosa
  • Species tree

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Multi-locus tree and species tree approaches toward resolving a complex clade of downy mildews (Straminipila, Oomycota), including pathogens of beet and spinach. / Choi, Young Joon; Klosterman, Steven J.; Kummer, Volker; Voglmayr, Hermann; Shin, Hyeon-Dong; Thines, Marco.

In: Molecular Phylogenetics and Evolution, Vol. 86, 01.05.2015, p. 24-34.

Research output: Contribution to journalArticle

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