Multi-order dynamic range DNA sensor using a gold decorated SWCNT random network

Jung Woo Ko, Jun Myung Woo, Ahn Jinhong, Jun Ho Cheon, Jae Heung Lim, Seok Hyang Kim, Honggu Chun, Eunhye Kim, Young June Park

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A novel electrical DNA biosensor is presented, which consists of gold (Au) nanoscale islands and a single-walled carbon nanotube (SWCNT) network on top of a concentric Au electrode array (also referred to as the CGi). The decorated Au islands on the SWCNT network provide ideal docking sites for ss-DNA probe (p-DNA) molecules. They also provide better adhesion between the SWCNT network and the chip substrate. In addition, the concentric electrode gives asymmetric current voltage characteristics in the solution and provides more flexible bias options to the electrodes. The sensor system is applied to a DNA sensor after functionalization with a 25mer p-DNA (5?-HSC6-C18- GCCATTCTCACCGGATTCAGTCGTC-3?), hereafter called the [CGi+p-DNA]. The response of the DNA sensor has been measured in both real-time during hybridization with the complementary target ss-DNAs (t-DNA) and the static mode after the hybridization and washing steps. A wide dynamic range from the 100 fM to 1 μM has been achieved from the real-time mode and the static mode. Moreover, it is shown that the sensor system differentiates partially mismatched (single nucleotide polymorphism (SNP), half mismatch, noncomplementary) t-DNA, as well. The [CGi] sensor platform can be easily extended to target specific biological recognition elements such as aptamers or proteins.

Original languageEnglish
Pages (from-to)4365-4372
Number of pages8
JournalACS Nano
Volume5
Issue number6
DOIs
Publication statusPublished - 2011 Jun 28

Keywords

  • CNT FET
  • DNA sensor
  • dynamic range
  • electrical biosensor
  • gold islands
  • random network
  • single-walled carbon nanotube
  • SNP

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

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  • Cite this

    Ko, J. W., Woo, J. M., Jinhong, A., Cheon, J. H., Lim, J. H., Kim, S. H., Chun, H., Kim, E., & Park, Y. J. (2011). Multi-order dynamic range DNA sensor using a gold decorated SWCNT random network. ACS Nano, 5(6), 4365-4372. https://doi.org/10.1021/nn102938h