Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS

T. T N Lien, Tran Dai Lam, V. T H An, Tran Vinh Hoang, Duong Tuan Quang, Dinh Quang Khieu, Toshifumi Tsukahara, Young Hoon Lee, Jong Seung Kim

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59 Citations (Scopus)

Abstract

In this paper, we describe DNA electrochemical detection for genetically modified organism (GMO) based on multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole (PPy). DNA hybridization is studied by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). An increase in DNA complementary target concentration results in a decrease in the faradic charge transfer resistance (Rct) and signifying "signal-on" behavior of MWCNTs-PPy-DNA system. QCM and EIS data indicated that the electroanalytical MWCNTs-PPy films were highly sensitive (as low as 4 pM of target can be detected with QCM technique). In principle, this system can be suitable not only for DNA but also for protein biosensor construction.

Original languageEnglish
Pages (from-to)1164-1169
Number of pages6
JournalTalanta
Volume80
Issue number3
DOIs
Publication statusPublished - 2010 Jan 1

Keywords

  • Electrochemical DNA biosensor
  • Electrochemical impedance spectroscopy
  • MWCNTs-doped polypyrrole
  • Quartz crystal microbalance

ASJC Scopus subject areas

  • Chemistry(all)

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  • Cite this

    Lien, T. T. N., Lam, T. D., An, V. T. H., Hoang, T. V., Quang, D. T., Khieu, D. Q., Tsukahara, T., Lee, Y. H., & Kim, J. S. (2010). Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS. Talanta, 80(3), 1164-1169. https://doi.org/10.1016/j.talanta.2009.09.002