Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS

T. T N Lien, Tran Dai Lam, V. T H An, Tran Vinh Hoang, Duong Tuan Quang, Dinh Quang Khieu, Toshifumi Tsukahara, Young Hoon Lee, Jong Seung Kim

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

In this paper, we describe DNA electrochemical detection for genetically modified organism (GMO) based on multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole (PPy). DNA hybridization is studied by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). An increase in DNA complementary target concentration results in a decrease in the faradic charge transfer resistance (Rct) and signifying "signal-on" behavior of MWCNTs-PPy-DNA system. QCM and EIS data indicated that the electroanalytical MWCNTs-PPy films were highly sensitive (as low as 4 pM of target can be detected with QCM technique). In principle, this system can be suitable not only for DNA but also for protein biosensor construction.

Original languageEnglish
Pages (from-to)1164-1169
Number of pages6
JournalTalanta
Volume80
Issue number3
DOIs
Publication statusPublished - 2010 Jan 1

Fingerprint

Quartz Crystal Microbalance Techniques
Genetically Modified Organisms
Dielectric Spectroscopy
Carbon Nanotubes
Quartz crystal microbalances
Biosensing Techniques
Electrochemical impedance spectroscopy
Biosensors
Labels
DNA
Charge transfer
Complementary DNA
polypyrrole
Proteins

Keywords

  • Electrochemical DNA biosensor
  • Electrochemical impedance spectroscopy
  • MWCNTs-doped polypyrrole
  • Quartz crystal microbalance

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS. / Lien, T. T N; Lam, Tran Dai; An, V. T H; Hoang, Tran Vinh; Quang, Duong Tuan; Khieu, Dinh Quang; Tsukahara, Toshifumi; Lee, Young Hoon; Kim, Jong Seung.

In: Talanta, Vol. 80, No. 3, 01.01.2010, p. 1164-1169.

Research output: Contribution to journalArticle

Lien, T. T N ; Lam, Tran Dai ; An, V. T H ; Hoang, Tran Vinh ; Quang, Duong Tuan ; Khieu, Dinh Quang ; Tsukahara, Toshifumi ; Lee, Young Hoon ; Kim, Jong Seung. / Multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole DNA biosensor for label-free detection of genetically modified organisms by QCM and EIS. In: Talanta. 2010 ; Vol. 80, No. 3. pp. 1164-1169.
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AU - Lien, T. T N

AU - Lam, Tran Dai

AU - An, V. T H

AU - Hoang, Tran Vinh

AU - Quang, Duong Tuan

AU - Khieu, Dinh Quang

AU - Tsukahara, Toshifumi

AU - Lee, Young Hoon

AU - Kim, Jong Seung

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