Multi-well chip for forming a uniform embryoid body in a tiny droplet with mouse embryonic stem cells

Choong Kim; In Hye Lee; Kangsun Lee; Sung Shin Ryu; Sang Ho Lee; Kyu Jung Lee; Jinwoo Lee; Ji Yoon Kang; Tae Song Kim

doi:10.1271/bbb.70384

Multi-well chip for forming a uniform embryoid body in a tiny droplet with mouse embryonic stem cells

Choong Kim, In Hye Lee, Kangsun Lee, Sung Shin Ryu, Sang Ho Lee, Kyu Jung Lee, Jinwoo Lee, Ji Yoon Kang, Tae Song Kim

* Honorary professors

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A multi-well chip (MWC) is described by which mouse embryonic carcinoma (EC) stem cells form a comparatively more rapid and uniform embryoid body (EB) over the conventional hanging drop (HD) method. The newly developed MWC consists of an array of extruded through-holes, each of which holds a droplet of the cell suspension. The study found that the small curvature radius of the droplet in the MWC improved the EB formation rate of a hanging drop from 70% to 98%. Furthermore, the EBs formed by the MWC were uniformly round in shape regardless of the number of suspended cells ranging from 0.5 × 10³ to 20 × 10³. The ratio of beating colonies from the MWC was over 2-fold larger than that from HD. The experiments demonstrate that the MWC will be a valuable experimental tool for robust and reproducible EB-based differentiation of a defined number of ES cells.

Original language	English
Pages (from-to)	2985-2991
Number of pages	7
Journal	Bioscience, Biotechnology and Biochemistry
Volume	71
Issue number	12
DOIs	https://doi.org/10.1271/bbb.70384
Publication status	Published - 2007

Keywords

Differentiation
Embryoid body
Hanging drop
Multi-well chip

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.70384

Cite this

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title = "Multi-well chip for forming a uniform embryoid body in a tiny droplet with mouse embryonic stem cells",

abstract = "A multi-well chip (MWC) is described by which mouse embryonic carcinoma (EC) stem cells form a comparatively more rapid and uniform embryoid body (EB) over the conventional hanging drop (HD) method. The newly developed MWC consists of an array of extruded through-holes, each of which holds a droplet of the cell suspension. The study found that the small curvature radius of the droplet in the MWC improved the EB formation rate of a hanging drop from 70% to 98%. Furthermore, the EBs formed by the MWC were uniformly round in shape regardless of the number of suspended cells ranging from 0.5 × 103 to 20 × 103. The ratio of beating colonies from the MWC was over 2-fold larger than that from HD. The experiments demonstrate that the MWC will be a valuable experimental tool for robust and reproducible EB-based differentiation of a defined number of ES cells.",

keywords = "Differentiation, Embryoid body, Hanging drop, Multi-well chip",

author = "Choong Kim and Lee, {In Hye} and Kangsun Lee and Ryu, {Sung Shin} and Lee, {Sang Ho} and Lee, {Kyu Jung} and Jinwoo Lee and Kang, {Ji Yoon} and Kim, {Tae Song}",

note = "Funding Information: This research was supported by a KIST grant and the Intelligent Microsystem Center (IMC; http://www. microsystem.re.kr)-which is carrying out one of the 21st century{\textquoteright}s frontier R&D projects sponsored by the Korea Ministry of Commerce, Industry and Energy.",

year = "2007",

doi = "10.1271/bbb.70384",

language = "English",

volume = "71",

pages = "2985--2991",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "12",

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T1 - Multi-well chip for forming a uniform embryoid body in a tiny droplet with mouse embryonic stem cells

AU - Kim, Choong

AU - Lee, In Hye

AU - Lee, Kangsun

AU - Ryu, Sung Shin

AU - Lee, Sang Ho

AU - Lee, Kyu Jung

AU - Lee, Jinwoo

AU - Kang, Ji Yoon

AU - Kim, Tae Song

N1 - Funding Information: This research was supported by a KIST grant and the Intelligent Microsystem Center (IMC; http://www. microsystem.re.kr)-which is carrying out one of the 21st century’s frontier R&D projects sponsored by the Korea Ministry of Commerce, Industry and Energy.

PY - 2007

Y1 - 2007

N2 - A multi-well chip (MWC) is described by which mouse embryonic carcinoma (EC) stem cells form a comparatively more rapid and uniform embryoid body (EB) over the conventional hanging drop (HD) method. The newly developed MWC consists of an array of extruded through-holes, each of which holds a droplet of the cell suspension. The study found that the small curvature radius of the droplet in the MWC improved the EB formation rate of a hanging drop from 70% to 98%. Furthermore, the EBs formed by the MWC were uniformly round in shape regardless of the number of suspended cells ranging from 0.5 × 103 to 20 × 103. The ratio of beating colonies from the MWC was over 2-fold larger than that from HD. The experiments demonstrate that the MWC will be a valuable experimental tool for robust and reproducible EB-based differentiation of a defined number of ES cells.

AB - A multi-well chip (MWC) is described by which mouse embryonic carcinoma (EC) stem cells form a comparatively more rapid and uniform embryoid body (EB) over the conventional hanging drop (HD) method. The newly developed MWC consists of an array of extruded through-holes, each of which holds a droplet of the cell suspension. The study found that the small curvature radius of the droplet in the MWC improved the EB formation rate of a hanging drop from 70% to 98%. Furthermore, the EBs formed by the MWC were uniformly round in shape regardless of the number of suspended cells ranging from 0.5 × 103 to 20 × 103. The ratio of beating colonies from the MWC was over 2-fold larger than that from HD. The experiments demonstrate that the MWC will be a valuable experimental tool for robust and reproducible EB-based differentiation of a defined number of ES cells.

KW - Differentiation

KW - Embryoid body

KW - Hanging drop

KW - Multi-well chip

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DO - 10.1271/bbb.70384

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AN - SCOPUS:37649016589

SN - 0916-8451

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 12

ER -

Multi-well chip for forming a uniform embryoid body in a tiny droplet with mouse embryonic stem cells

Abstract

Keywords

ASJC Scopus subject areas

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