Multi-well chip for forming a uniform embryoid body in a tiny droplet with mouse embryonic stem cells

Choong Kim, In Hye Lee, Kangsun Lee, Sung Shin Ryu, Sang Ho Lee, Kyu Jung Lee, Jinwoo Lee, Ji Yoon Kang, Tae Song Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A multi-well chip (MWC) is described by which mouse embryonic carcinoma (EC) stem cells form a comparatively more rapid and uniform embryoid body (EB) over the conventional hanging drop (HD) method. The newly developed MWC consists of an array of extruded through-holes, each of which holds a droplet of the cell suspension. The study found that the small curvature radius of the droplet in the MWC improved the EB formation rate of a hanging drop from 70% to 98%. Furthermore, the EBs formed by the MWC were uniformly round in shape regardless of the number of suspended cells ranging from 0.5 × 103 to 20 × 103. The ratio of beating colonies from the MWC was over 2-fold larger than that from HD. The experiments demonstrate that the MWC will be a valuable experimental tool for robust and reproducible EB-based differentiation of a defined number of ES cells.

Original languageEnglish
Pages (from-to)2985-2991
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number12
DOIs
Publication statusPublished - 2007 Dec 1

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Embryoid Bodies
embryonic stem cells
Stem cells
droplets
mice
Cell Count
cell suspension culture
carcinoma
stem cells
cells
Suspensions
Carcinoma
Mouse Embryonic Stem Cells
methodology
Experiments

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Multi-well chip for forming a uniform embryoid body in a tiny droplet with mouse embryonic stem cells. / Kim, Choong; Lee, In Hye; Lee, Kangsun; Ryu, Sung Shin; Lee, Sang Ho; Lee, Kyu Jung; Lee, Jinwoo; Kang, Ji Yoon; Kim, Tae Song.

In: Bioscience, Biotechnology and Biochemistry, Vol. 71, No. 12, 01.12.2007, p. 2985-2991.

Research output: Contribution to journalArticle

Kim, Choong ; Lee, In Hye ; Lee, Kangsun ; Ryu, Sung Shin ; Lee, Sang Ho ; Lee, Kyu Jung ; Lee, Jinwoo ; Kang, Ji Yoon ; Kim, Tae Song. / Multi-well chip for forming a uniform embryoid body in a tiny droplet with mouse embryonic stem cells. In: Bioscience, Biotechnology and Biochemistry. 2007 ; Vol. 71, No. 12. pp. 2985-2991.
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