Multicomponent, peptide-targeted glycol chitosan nanoparticles containing ferrimagnetic iron oxide nanocubes for bladder cancer multimodal imaging

Jaehong Key, Deepika Dhawan, Christy L. Cooper, Deborah W. Knapp, Kwang Meyung Kim, Ick Chan Kwon, Kuiwon Choi, Kinam Park, Paolo Decuzzi, James F. Leary

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

While current imaging modalities, such as magnetic resonance imaging (MRI), computed tomography, and positron emission tomography, play an important role in detecting tumors in the body, no single-modality imaging possesses all the functions needed for a complete diagnostic imaging, such as spatial resolution, signal sensitivity, and tissue penetration depth. For this reason, multimodal imaging strategies have become promising tools for advanced biomedical research and cancer diagnostics and therapeutics. In designing multimodal nanoparticles, the physicochemical properties of the nanoparticles should be engineered so that they successfully accumulate at the tumor site and minimize nonspecific uptake by other organs. Finely altering the nano-scale properties can dramatically change the biodistribution and tumor accumulation of nanoparticles in the body. In this study, we engineered multimodal nanoparticles for both MRI, by using ferrimagnetic nanocubes (NCs), and near infrared fluorescence imaging, by using cyanine 5.5 fluorescence molecules. We changed the physicochemical properties of glycol chitosan nanoparticles by conjugating bladder cancer-targeting peptides and loading many ferrimagnetic iron oxide NCs per glycol chitosan nanoparticle to improve MRI contrast. The 22 nm ferrimagnetic NCs were stabilized in physiological conditions by encapsulating them within modified chitosan nanoparticles. The multimodal nanoparticles were compared with in vivo MRI and near infrared fluorescent systems. We demonstrated significant and important changes in the biodistribution and tumor accumulation of nanoparticles with different physicochemical properties. Finally, we demonstrated that multimodal nanoparticles specifically visualize small tumors and show minimal accumulation in other organs. This work reveals the importance of finely modulating physicochemical properties in designing multimodal nanoparticles for bladder cancer imaging.

Original languageEnglish
Pages (from-to)4141-4155
Number of pages15
JournalInternational Journal of Nanomedicine
Volume11
DOIs
Publication statusPublished - 2016 Aug 29
Externally publishedYes

Fingerprint

Multimodal Imaging
Glycols
Iron oxides
Chitosan
Urinary Bladder Neoplasms
Nanoparticles
Peptides
Imaging techniques
Tumors
Magnetic resonance
Magnetic Resonance Imaging
Neoplasms
ferric oxide
glycol-chitosan
Fluorescence
Infrared radiation
Positron emission tomography
Optical Imaging
Diagnostic Imaging
Tomography

Keywords

  • Bladder cancer
  • Chitosan
  • Iron oxide
  • MRI
  • Multimodal imaging
  • NIRF

ASJC Scopus subject areas

  • Bioengineering
  • Biophysics
  • Biomaterials
  • Drug Discovery
  • Organic Chemistry

Cite this

Multicomponent, peptide-targeted glycol chitosan nanoparticles containing ferrimagnetic iron oxide nanocubes for bladder cancer multimodal imaging. / Key, Jaehong; Dhawan, Deepika; Cooper, Christy L.; Knapp, Deborah W.; Kim, Kwang Meyung; Kwon, Ick Chan; Choi, Kuiwon; Park, Kinam; Decuzzi, Paolo; Leary, James F.

In: International Journal of Nanomedicine, Vol. 11, 29.08.2016, p. 4141-4155.

Research output: Contribution to journalArticle

Key, Jaehong ; Dhawan, Deepika ; Cooper, Christy L. ; Knapp, Deborah W. ; Kim, Kwang Meyung ; Kwon, Ick Chan ; Choi, Kuiwon ; Park, Kinam ; Decuzzi, Paolo ; Leary, James F. / Multicomponent, peptide-targeted glycol chitosan nanoparticles containing ferrimagnetic iron oxide nanocubes for bladder cancer multimodal imaging. In: International Journal of Nanomedicine. 2016 ; Vol. 11. pp. 4141-4155.
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