Multifunctional fluorescent nanoprobe for sequential detections of Hg2+ Ions and biothiols in live cells

Lu He, Hui Tao, Seyoung Koo, Guo Chen, Amit Sharma, Yong Chen, In Taek Lim, Qian Yong Cao, Jong Seung Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Herein, we report an amphiphilic fluorescent probe consisting of a dansyl fluorophore as a reporter and a hydrophobic cetyl chain bridged by a triazole unit. The cetyl-based probe can self-assemble to form nanoaggregates in aqueous solution, as confirmed by Tyndall effect, dynamic light scattering (DLS), and transmission electron microscopy (TEM) measurements. This probe exhibited an "on-off" fluorescence quenching response toward Hg2+ ions in aqueous solution over other tested metal ions. In contrast, the analogous methyl-based probe barely exhibits Hg2+ ion sensing behavior under the same conditions. Moreover, the resulting complex of the cetyl-based probe and Hg2+ (1-Hg2+, 1:1 stoichiometry) exhibited an efficient fluorescence "off-on" sensing for thiol-containing amino acids, including cysteine (Cys), homocysteine (Hcy), and glutathione (GSH). This nanoprobe exhibited minimal cytotoxicity with excellent cell permeability and was efficiently tested for the imaging of intracellular Hg2+ and cysteine in live cells.

Original languageEnglish
Pages (from-to)871-878
Number of pages8
JournalACS Applied Bio Materials
Volume1
Issue number3
DOIs
Publication statusPublished - 2018 Jan 1

Fingerprint

Nanoprobes
Ions
Cysteine
Fluorescence
Triazoles
Homocysteine
Transmission Electron Microscopy
Fluorescent Dyes
Sulfhydryl Compounds
Glutathione
Fluorophores
Permeability
Dynamic light scattering
Cytotoxicity
Light transmission
Metals
Stoichiometry
Metal ions
Amino Acids
Quenching

Keywords

  • Fluorescence imaging
  • Mercury sensing
  • Nanoaggregates
  • Thiols recognition

ASJC Scopus subject areas

  • Biomaterials
  • Chemistry(all)
  • Biomedical Engineering
  • Biochemistry, medical

Cite this

Multifunctional fluorescent nanoprobe for sequential detections of Hg2+ Ions and biothiols in live cells. / He, Lu; Tao, Hui; Koo, Seyoung; Chen, Guo; Sharma, Amit; Chen, Yong; Lim, In Taek; Cao, Qian Yong; Kim, Jong Seung.

In: ACS Applied Bio Materials, Vol. 1, No. 3, 01.01.2018, p. 871-878.

Research output: Contribution to journalArticle

He, Lu ; Tao, Hui ; Koo, Seyoung ; Chen, Guo ; Sharma, Amit ; Chen, Yong ; Lim, In Taek ; Cao, Qian Yong ; Kim, Jong Seung. / Multifunctional fluorescent nanoprobe for sequential detections of Hg2+ Ions and biothiols in live cells. In: ACS Applied Bio Materials. 2018 ; Vol. 1, No. 3. pp. 871-878.
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