Multifunctional fluorescent nanoprobe for sequential detections of Hg2+ Ions and biothiols in live cells

Lu He, Hui Tao, Seyoung Koo, Guo Chen, Amit Sharma, Yong Chen, In Taek Lim, Qian Yong Cao, Jong Seung Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Herein, we report an amphiphilic fluorescent probe consisting of a dansyl fluorophore as a reporter and a hydrophobic cetyl chain bridged by a triazole unit. The cetyl-based probe can self-assemble to form nanoaggregates in aqueous solution, as confirmed by Tyndall effect, dynamic light scattering (DLS), and transmission electron microscopy (TEM) measurements. This probe exhibited an "on-off" fluorescence quenching response toward Hg2+ ions in aqueous solution over other tested metal ions. In contrast, the analogous methyl-based probe barely exhibits Hg2+ ion sensing behavior under the same conditions. Moreover, the resulting complex of the cetyl-based probe and Hg2+ (1-Hg2+, 1:1 stoichiometry) exhibited an efficient fluorescence "off-on" sensing for thiol-containing amino acids, including cysteine (Cys), homocysteine (Hcy), and glutathione (GSH). This nanoprobe exhibited minimal cytotoxicity with excellent cell permeability and was efficiently tested for the imaging of intracellular Hg2+ and cysteine in live cells.

Original languageEnglish
Pages (from-to)871-878
Number of pages8
JournalACS Applied Bio Materials
Volume1
Issue number3
DOIs
Publication statusPublished - 2018 Jan 1

Keywords

  • Fluorescence imaging
  • Mercury sensing
  • Nanoaggregates
  • Thiols recognition

ASJC Scopus subject areas

  • Biomaterials
  • Chemistry(all)
  • Biomedical Engineering
  • Biochemistry, medical

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