TY - JOUR
T1 - Multigene phylogenies of Ophiostoma clavigerum and closely related species from bark beetle-attacked Pinus in North America
AU - Lim, Young Woon
AU - Alamouti, Sepideh Massoumi
AU - Kim, Jae Jin
AU - Lee, Sangwon
AU - Breuil, Colette
N1 - Funding Information:
This work was supported by the Natural Sciences and Engineering Research Council of Canada. We thank Drs. D.L. Six, M.J. Wingfield, and A. Uzunovic for providing cultures. We thank Julie Yi (Macrogen) and Monica Lu for technical assistance.
PY - 2004/8/1
Y1 - 2004/8/1
N2 - Leptographium pyrinum, Leptographium terebrantis, Ophiostoma aureum, Ophiostoma clavigerum, and Ophiostoma robustum are very similar in morphology, host trees choice, and the way they are disseminated by bark beetles. Their phylogenetic relationships were clarified using rDNA and protein coding genes including actin, β-tubulin, and translation elongation factor-1α. Protein coding gene trees showed better resolution than the rDNA tree, which generated three clades: O. clavigerum, L. terebrantis/L. pyrinum, and O. robustum/O. aureum. A combined gene phylogenetic tree, which was supported by high bootstrap values, showed that O. aureum, L. pyrinum, O. robustum, and O. clavigerum each formed distinct clades while L. terebrantis was paraphyletic to O. clavigerum. The higher variability of the protein coding genes and the congruity in their phylogenetic results suggested that these genes may be better markers for identifying closely related species. These gene trees have also facilitated the description of the evolutionary relationships among these species.
AB - Leptographium pyrinum, Leptographium terebrantis, Ophiostoma aureum, Ophiostoma clavigerum, and Ophiostoma robustum are very similar in morphology, host trees choice, and the way they are disseminated by bark beetles. Their phylogenetic relationships were clarified using rDNA and protein coding genes including actin, β-tubulin, and translation elongation factor-1α. Protein coding gene trees showed better resolution than the rDNA tree, which generated three clades: O. clavigerum, L. terebrantis/L. pyrinum, and O. robustum/O. aureum. A combined gene phylogenetic tree, which was supported by high bootstrap values, showed that O. aureum, L. pyrinum, O. robustum, and O. clavigerum each formed distinct clades while L. terebrantis was paraphyletic to O. clavigerum. The higher variability of the protein coding genes and the congruity in their phylogenetic results suggested that these genes may be better markers for identifying closely related species. These gene trees have also facilitated the description of the evolutionary relationships among these species.
KW - Actin
KW - Bark beetle
KW - Multigene phylogenies
KW - Ophiostoma clavigerum
KW - Translation elongation factor 1α
KW - rDNA
KW - β-Tubulin
UR - http://www.scopus.com/inward/record.url?scp=3242778597&partnerID=8YFLogxK
U2 - 10.1016/j.femsle.2004.06.020
DO - 10.1016/j.femsle.2004.06.020
M3 - Article
C2 - 15268942
AN - SCOPUS:3242778597
SN - 0378-1097
VL - 237
SP - 89
EP - 96
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -