Multigene phylogenies of Ophiostoma clavigerum and closely related species from bark beetle-attacked Pinus in North America

Young Woon Lim, Sepideh Massoumi Alamouti, Jae-Jin Kim, Sangwon Lee, Colette Breuil

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Leptographium pyrinum, Leptographium terebrantis, Ophiostoma aureum, Ophiostoma clavigerum, and Ophiostoma robustum are very similar in morphology, host trees choice, and the way they are disseminated by bark beetles. Their phylogenetic relationships were clarified using rDNA and protein coding genes including actin, β-tubulin, and translation elongation factor-1α. Protein coding gene trees showed better resolution than the rDNA tree, which generated three clades: O. clavigerum, L. terebrantis/L. pyrinum, and O. robustum/O. aureum. A combined gene phylogenetic tree, which was supported by high bootstrap values, showed that O. aureum, L. pyrinum, O. robustum, and O. clavigerum each formed distinct clades while L. terebrantis was paraphyletic to O. clavigerum. The higher variability of the protein coding genes and the congruity in their phylogenetic results suggested that these genes may be better markers for identifying closely related species. These gene trees have also facilitated the description of the evolutionary relationships among these species.

Original languageEnglish
Pages (from-to)89-96
Number of pages8
JournalFEMS Microbiology Letters
Volume237
Issue number1
DOIs
Publication statusPublished - 2004 Aug 1
Externally publishedYes

Fingerprint

Ophiostoma
Pinus
Beetles
Phylogeny
North America
Ribosomal DNA
Genes
Peptide Elongation Factor 1
Proteins
Tubulin
Actins

Keywords

  • β-Tubulin
  • Actin
  • Bark beetle
  • Multigene phylogenies
  • Ophiostoma clavigerum
  • rDNA
  • Translation elongation factor 1α

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Multigene phylogenies of Ophiostoma clavigerum and closely related species from bark beetle-attacked Pinus in North America. / Lim, Young Woon; Alamouti, Sepideh Massoumi; Kim, Jae-Jin; Lee, Sangwon; Breuil, Colette.

In: FEMS Microbiology Letters, Vol. 237, No. 1, 01.08.2004, p. 89-96.

Research output: Contribution to journalArticle

Lim, Young Woon ; Alamouti, Sepideh Massoumi ; Kim, Jae-Jin ; Lee, Sangwon ; Breuil, Colette. / Multigene phylogenies of Ophiostoma clavigerum and closely related species from bark beetle-attacked Pinus in North America. In: FEMS Microbiology Letters. 2004 ; Vol. 237, No. 1. pp. 89-96.
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